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SCT068 BioTracker DUAL dUTPase Live Cell reporter

Live cell probe for activity of cellular enzyme deoxyuridine triphosphatase
(dUTPase) involved in deactivation of damaged nucleotides. The probe acts as a luciferase luminescence
reporter of dUPTase.

SCT068
1 mg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberSCT068
DescriptionBioTracker DUAL dUTPase Live Cell reporter
OverviewNucleotide surveillance enzymes play important roles in
human health, by monitoring damaged monomers in the nucleotide pool
and deactivating them before they are incorporated into chromosomal
DNA or disrupt nucleotide metabolism. Deamination of
cytosine, leading to uracil in DNA and in the nucleotide pool, can be
deleterious, causing DNA damage. The enzyme deoxyuridine triphosphatase
(dUTPase) is the sole enzyme that cleanses the nucleotide pool of deaminated cytosine. Measuring the activity of this enzyme is important
both in basic research and in clinical applications involving this
pathway.

The BioTracker DUAL dUTPase live cell probe is a luminescence probe that specifically reports on dUTPase activity in real time. The probe is a chimera of dUTP and ATP. dUTPase enzyme accepts the DUAL probe as an efficient substrate and upon hydrolysis releases ATP, which is detected sensitively by luciferase luminescence.

Note: Luceferin and luciferase enzyme are needed to detect ATP released by hydrolysis, but are not included in this product.

Spectral Properties
Luminescence intensity.

References
Ji D et al. ATP-Linked Chimeric Nucleotide as a Specific Luminescence Reporter
of Deoxyuridine Triphosphatase. Bioconjugate Chem. 2018, 29, 1614-1621.
Alternate Names
  • Live cell imaging probe
References
Product Information
Detection methodChemiluminescence
PresentationLyophilized
Applications
ApplicationLive cell probe for activity of cellular enzyme deoxyuridine triphosphatase
(dUTPase) involved in deactivation of damaged nucleotides. The probe acts as a luciferase luminescence
reporter of dUPTase.
Key Applications
  • Cell Based Assays
  • Activity Assay
Application NotesLive cell fluorescent imaging
Biological Information
ConcentrationPlease refer to lot specific datasheet.
Molecular Weight865.5 g/mol
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssurancePurity: ≥ 98% confirmed by LCMS. HNMR, PNMR and LC-MS confirms structure. Molar Mass: 865.5 g/mol
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker DUAL dUTPase Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
SCT068 04061841078104

Documentation