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SCT079 BioTracker CoxFluor Live Cell Dye

Fluorescent probe for monitoring COX-2 activity in live cells by confocal microscopy or flow cytometry.

SCT079
1 mg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberSCT079
DescriptionBioTracker CoxFluor Live Cell Dye
OverviewCyclooxygenase (COX), also known as Prostaglandin-endoperoxide synthase (PTGS), is the key enzyme in prostaglandin biosynthesis. There are two isozymes of cyclooxygenase: a constitutive COX-1 and an inducible COX-2, which differ in their regulation of expression and tissue distribution. COX-2 overexpression is prominent in inflammatory diseases, neurodegenerative disorders, and cancer. Directly monitoring COX-2 activity within its native environment poses an exciting approach to investigate the effect of the local environments on protein activity (1).

BioTracker CoxFluor Live Cell Dye is an isoform selective activity-based sensor for COX-2. The probe may be used for COX-2 inhibitor screening assays and for monitoring COX-2 activity in live cells by confocal microscopy or flow cytometry. CoxFluor strategically links a natural substrate with a dye precursor to engage both the cyclooxygenase and peroxidase activities of COX-2. This catalyzes the release of resorufin and the natural product, as supported by molecular dynamics and ensemble docking. CoxFluor enabled the detection of oxygen dependent changes in COX-2 activity that are independent of protein expression within live macrophage cells (1).

Spectral Properties
Absorbance: 540 nm
Emission: 590 nm

Reference:
1. Yadav, A. K., Reinhardt, C. J., Arango, A. S., Huff, H. C., Dong, L., Malkowski, M. G., ... & Chan, J. (2020). An Activity‐Based Sensing Approach for the Detection of Cyclooxygenase‐2 in Live Cells. Angewandte Chemie, 132(8), 3333-3340.
Alternate Names
  • Live Cell Imaging Probe
References
Product Information
Detection methodFluorescence
PresentationLyophilized. Off-white solid.
Applications
ApplicationFluorescent probe for monitoring COX-2 activity in live cells by confocal microscopy or flow cytometry.
Key Applications
  • Cell Based Assays
Application NotesLive cell fluorescent imaging
Biological Information
Molecular Weight501.66
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssurancePurity ≥ 95% confirmed by HPLC. Identification confirmed by HNMR, LC-MS, and elemental analysis.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
SCT079 04061841827993

Documentation