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GF156 B18R, Human Recombinant Carrier-Free

B18R protein can be used with the Simplicon® RNA Reprogramming Technology (Catalog No. SCR550) to create “foot-print” free human iPS cells at the highest efficiency without unwanted RNA degradation caused by cellular IFN responses.

B18R, Human Recombinant Carrier-Free MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture, Stem Cell Culture
GF156
50 µg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Key ApplicationsSpeciesSourcePurity
CULT, CultHuman Insect cells infected with baculovirus: amino acids His20-Glu 351 with a His-tag attached of Vaccinia Virus B18R.Product is greater than 98% pure, as determined by SDS-PAGE.
Product is less than 0.05 ng/ug cytokine as determined by the LAL assay
Description
Catalogue NumberGF156
DescriptionB18R, Human Recombinant Carrier-Free
Background InformationB18R is a virally-encoded protein that acts as a decoy receptor for Type 1 Interferons. B18R enables increased cell viability during RNA transfection protocols designed to convert human somatic donor cells into iPSCs via direct delivery of synthetic mRNA's. As a type I IFN receptor, B18R has a broad species specificity. It has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta.
References
Product Information
HS Code3504 00 90
PresentationSterile liquid;10 mM phosphate, 0.5 M NaCl, pH 7.5 buffer and is filtered with a 0.2µm filter before vialing.
Quality LevelMQ100
Applications
ApplicationB18R protein can be used with the Simplicon® RNA Reprogramming Technology (Catalog No. SCR550) to create “foot-print” free human iPS cells at the highest efficiency without unwanted RNA degradation caused by cellular IFN responses.
Key Applications
  • Cell Culture
  • Stem Cell Culture
Biological Information
Concentration0.5 mg/mL
PurityProduct is greater than 98% pure, as determined by SDS-PAGE.
Product is less than 0.05 ng/ug cytokine as determined by the LAL assay
SourceInsect cells infected with baculovirus: amino acids His20-Glu 351 with a His-tag attached of Vaccinia Virus B18R.
Gene Symbol
  • B18R
Stem Cell Type
  • Induced Pluripotent Stem Cells
Molecular WeightProduct has a predicted molecular mass of 38,389. The DTT-reduced protein migrates as a 48 kDa polypeptide on SDS-PAGE due to glycosylation. The non-reduced protein migrates as a 46 kDa polypeptide on SDS-PAGE due to internal cystines.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsFor best recovery, quick-spin vial prior to opening. Thaw on ice. While on ice, aliquot B18R protein into sterile, nuclease-free, low protein-binding eppendorf tubes and store at -80°C. Limit repeated freeze and thaw cycle. Use in a sterile environment.
Further dilutions should be made into appropriate buffer or medium.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
GF156 04055977116397

Documentation

B18R, Human Recombinant Carrier-Free MSDS

Title

Safety Data Sheet (SDS) 

B18R, Human Recombinant Carrier-Free Certificates of Analysis

TitleLot Number
B18R, Human Recombinant -2817954 2817954
B18R, Human Recombinant Carrier-Free - 3584526 3584526
B18R, Human Recombinant Carrier-Free - 3694091 3694091
B18R, Human Recombinant Carrier-Free - 3874981 3874981
B18R, Human Recombinant Carrier-Free - 3942286 3942286
B18R, Human Recombinant Carrier-Free - Q2482392 Q2482392
B18R, Human Recombinant Carrier-Free - VP2211032 VP2211032
B18R, Human Recombinant Carrier-Free -2702570 2702570
B18R, Human Recombinant Carrier-Free -2704662 2704662
B18R, Human Recombinant Carrier-Free -VP1812052 VP1812052
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Data Sheet

Title
B18R, Human Recombinant Carrier-Free

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Product Families

Cytokines and Growth Factors

EMD Millipore offers a comprehensive range of highly purified bioactive cytokines and growth factors for use in human, mouse, and rat cell culture, stem cell differentiation and ELISA studies.
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Kategóriák

Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors