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SCR727 B18R-E3L Plasmid (human codon optimized for B18R and E3L)

To strongly suppress more interferon responses than single B18R to enable more expression of a Simplicon® RNA or regular mRNA(s).

B18R-E3L Plasmid (human codon optimized for B18R and E3L) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
SCR727
1 vial  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberSCR727
DescriptionB18R-E3L Plasmid (human codon optimized for B18R and E3L)
Background InformationSimplicon® is a novel system to effect immediate high sustained protein expression of the target gene(s) without the risk of integration. The technology employs a self-replicating RNA based on the Venezuelan equine encephalitis (VEE) genome. By nature of a synthetic and self-replicative RNA, interferon (IFN) responses occur at the transfection and replication of Simplicon® RNA. The IFN responses reduce the expression and lifetime of Simplicon® RNA. Therefore, it is important to suppress the IFN responses in Simplicon® System. B18R acts as a decoy receptor for Type I Interferons and inhibit IFN-alpha, beta, and omega and other Type I IFN receptors. When only employing B18R, some IFN-beta response still occurs. The vaccinia virus E3L gene encodes two double-stranded RNA binding proteins that suppressed IFN-beta response and improved the expression of Simplicon® RNA at RNA transfection and continuous cell culture. In product B18R-E3L plasmid (human codon optimized for B18R and E3L), B18R and E3L are provided as a polycistronic DNA that can be transcribed into a polycistronic mRNA to be co-transfected with Simplicon® RNA(s) for IFN suppression. For continuous expression, B18R protein is needed to supply with B18R-conditioned medium (B18R-CM) or recombinant protein.
References
Product Information
Applications
ApplicationTo strongly suppress more interferon responses than single B18R to enable more expression of a Simplicon® RNA or regular mRNA(s).
Application NotesB18R-E3L Plasmid (human codon optimized for B18R and E3L) is provided as a polycistronic DNA with both the B18R and E3L genes to more strongly suppress the interferon (IFN) responses that degrades Simplicon® RNA(s) or regular mRNA(s). Upon transcription, B18R RNA (E3L) works at single co-transfection with Simplicon® RNA or repeated co-transfection with regular mRNA(s). B18R RNA (E3L) can also be used for production of B18R-CM (B18R-conditioned medium).
Biological Information
ConcentrationPlease refer to lot specific datasheet.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsB18R-E3L Plasmid (Cat. # SCR727). One (1) vial containing 10 µL of Plasmid (1 µg/µL). Store at -20 °C
Packaging Information
Material Size1 vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
SCR727 04054839501760

Documentation

B18R-E3L Plasmid (human codon optimized for B18R and E3L) MSDS

Title

Safety Data Sheet (SDS) 

B18R-E3L Plasmid (human codon optimized for B18R and E3L) Certificates of Analysis

TitleLot Number
B18R-E3L Plasmid (human codon optimized for B18R and E3L) - 3394174 3394174
B18R-E3L Plasmid (human codon optimized for B18R and E3L) - 3403813 3403813
B18R-E3L Plasmid (human codon optimized for B18R and E3L) - 3488956 3488956

User Guides

Title
SimpliconTM Expression System: "Designing, Cloning and RNA Synthesis for Expression of Self-Replicative RNA"

Vector Sequence

Title
B18R-E3L Plasmid (human codon optimized for B18R and E3L)