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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABF118 Sigma-AldrichAnti-sTRAIL Antibody, clone III6F

This Anti-sTRAIL Antibody, clone III6F is validated for use in Flow Cytometry, IHC, Immunoprecipitation for the detection of sTRAIL.

Anti-sTRAIL Antibody, clone III6F MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Brochures

Research Category: Inflammation & Immunology Research Sub-Category: Apoptosis - Additional Gene Symbol: TNFSF10, APO2L, TRAIL UniProt Number: P50591

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Species Reactivity	Key Applications	Host	Format	Antibody Type
H	FC, IHC, IP	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABF118
Description	Anti-sTRAIL Antibody, clone III6F
Alternate Names	Tumor necrosis factor ligand superfamily member 10 Apo-2 ligand Apo-2L TNF-related apoptosis-inducing ligand Protein TRAIL CD253
Background Information	TNF-related apoptosis-inducing ligand (sTRAIL or Apo-2L) is the endogenous ligand for the ubiquitous pro-apoptotic TRAIL-R1 and TRAIL-R2 receptors. Upon ligand binding, TRAIL receptors form trimeric structures at the plasma membrane around a central zinc atom. The trimeric TRAIL receptors bind the FADD adaptor protein which in turn recruits caspase 8. TRAIL/APO-2L signals ultimately converge on caspase 3 and related proteins that promote cell death. Importantly, activated TRAIL receptors work cooperatively with death receptors 4 and 5 in transformed cells to induce caspase signaling, thereby preventing the proliferation of abnormal cells. TRAIL/ApO-2L has been implicated in pathophysiology of many disease processes, including cancer and autoimmune diseases.

References

Product Information
Format	Purified
Control	HeLa cells
Presentation	Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-sTRAIL Antibody, clone III6F is validated for use in Flow Cytometry, IHC, Immunoprecipitation for the detection of sTRAIL.
Key Applications	Flow Cytometry Immunohistochemistry Immunoprecipitation
Application Notes	Immunohistochemistry Analysis: A representative lot from an independent laboratory detected sTRAIL in frozen sections from human tonsils, lymph nodes and appendix (Liabakk, N. B., et al. (2002). J Immunol Methods. 259(1-2):119-128.). Flow Cytometry Analysis: A representative lot from an independent laboratory detected sTRAIL in REH cells (Liabakk, N. B., et al. (2002). J Immunol Methods. 259(1-2):119-128.).

Biological Information
Immunogen	Human soluble TRAIL
Clone	III6F
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Isotype	IgG2bκ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_003801
Entrez Gene Summary	The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene Symbol	TNFSF10 APO2L TRAIL
Purification Method	Protein G Purified
UniProt Number	P50591
UniProt Summary	FUNCTION: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. COFACTOR: Binds 1 zinc ion per trimer. SUBUNIT STRUCTURE: Homotrimer. SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein. TISSUE SPECIFICITY: Widespread; most predominant in spleen, lung and prostate. SEQUENCE SIMILARITIES: Belongs to the tumor necrosis factor family.
Molecular Weight	33 kDa calculated

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Flow Cytometry in HeLa cells. Flow Cytometry Analysis: 2.5 µg/mL of this antibody detected sTRAIL in 2X10E5 HeLa cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Katalógusszám	GTIN
MABF118	04053252596056

Documentation

Anti-sTRAIL Antibody, clone III6F MSDS

Title
Safety Data Sheet (SDS)

Anti-sTRAIL Antibody, clone III6F Certificates of Analysis

Title	Lot Number
Anti-sTRAIL, clone III6F - 2394057	2394057
Anti-sTRAIL, clone III6F - Q1998583	Q1998583
Anti-sTRAIL, clone III6F Monoclonal Antibody	3019781

References

Reference overview	Pub Med ID
Development, characterization and use of monoclonal antibodies against sTRAIL: measurement of sTRAIL by ELISA. Liabakk, Nina Beate, et al. J. Immunol. Methods, 259: 119-28 (2002) 2002 Kivonat megmutatása Two monoclonal antibodies against tumor necrosis factor (TNF)-related apoptosis inducing ligand (TRAIL), designated VI10E and III6F, have been generated. These antibodies were useful in flow cytometry analysis, immunohistochemistry, immunoprecipitation and in the development of an immunoassay for the detection of soluble TRAIL (sTRAIL)in biological samples. The immunoassay was based on two monoclonal antibodies against TRAIL. VI10E was used as the capture antibody and bound TRAIL was detected with anti-TRAIL from R&D Systems which was digoxigenin (DIG)-labeled. This enzyme-linked immunosorbent assay (ELISA) was specific for TRAIL since a panel of other cytokines did not affect the signal. The immunoassay was suitable for the detection of sTRAIL in human serum and plasma samples, cell culture supernatants and cell lysates. In a preliminary screening, it was found that serum samples from human immunodeficiency virus (HIV)-infected patients contained sTRAIL, and all these positive samples were found in the AIDS group. Using the immunoassay, it was found that phytohaemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (PBMC) to produce significant amounts of sTRAIL, the levels of which increased with exposure time. Thus, the immunoassay for TRAIL presented here represents a useful tool for measuring sTRAIL in various biological samples. It will also permit studies of release mechanisms as well as possible functions of the soluble form of this molecule.	11730847

Reference overview

Pub Med ID

Brochure

Title
New Products: Volume 3, 2012

Kapcsolódó termékek és alkalmazások

Katalógusszám	Leírás
MABC147	Anti-TRAIL/CD253 Antibody, clone 6D12.2

Kategóriák

Life Science Research > Antibodies and Assays > Primary Antibodies

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