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MABE1031 Anti-poly-ADP-ribose binding reagent

Anti-poly-ADP-ribose binding reagent is a reagent that selectively binds to ADP ribose for use in Western Blotting, Immunocytochemistry and Dot Blot.

Anti-poly-ADP-ribose binding reagent MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: General Post-translation Modification
MABE1031
200 µL  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormat
M, HWB, ICC, DBE. coliPurified
Description
Catalogue NumberMABE1031
DescriptionAnti-poly-ADP-ribose binding reagent
Alternate Names
  • poly-ADP-ribose binding reagent
Background InformationCat. No. MABE1031, Anti-poly-ADP-ribose binding reagent, is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta™(DE3)pLysS strain of E. coli (Cat. No. 70956). Anti-poly-ADP-ribose binding reagent is useful for the affinity detection of oligo- and poly-ADP-ribosylated (PARylated) proteins on membranes or on fixed cells in a manner similar to antibody-based Western blot, dot blot, and immunocytochemistry applications. The rabbit Fc tag allows visualization of the binding/labeling with conjugated anti-rabbit secondary antibodies. The Fc tag also allows Anti-poly-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications.
References
Product Information
FormatPurified
PresentationPurified from E. coli by Ni-NTA agarose. Supplied in buffer containing 10 mM Tris pH 7.5, 0.2 M NaCl, 10% Glycerol, 10 mM Imidazole, 1 mM PMSF, 1 mM β-Mercaptoethanol, 10% glycerol without preservatives.
Quality LevelMQ100
Applications
ApplicationAnti-poly-ADP-ribose binding reagent is a reagent that selectively binds to ADP ribose for use in Western Blotting, Immunocytochemistry and Dot Blot.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Dot Blot
Application NotesDot Blot Specificity Analysis: This reagent detected oligo(ADPR) and poly(ADPR) on ADP-ribosylated PARP1 recombinant protein (Lee Kraus, University of Texas Southwestern Medical Center).
Immunocytochemistry Analysis: A representative lot detected oligo(ADPR) and poly(ADPR)/PAR in 3T3-L1 cells (Lee Kraus, University of Texas Southwestern Medical Center).
Biological Information
ConcentrationPlease refer to lot specific datasheet.
HostE. coli
SpecificityTwo or more units of ADP-ribose
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteMouse, Human. Predicted to react with all species.
Purification MethodNi-NTA agarose
Molecular WeightVariable depending on the target proteins and the extend of ADP-ribosylation
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting on ADP-ribosylated PARP1 and PARP3 recombinant proteins.

Western Blotting Analysis: This reagent detected oligo(ADPR) and poly(ADPR) on ADP-ribosylated PARP1 recombinant protein (Lee Kraus, University of Texas Southwestern Medical Center).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -80°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABE1031 04055977168273

Documentation

Anti-poly-ADP-ribose binding reagent MSDS

Title

Safety Data Sheet (SDS) 

Anti-poly-ADP-ribose binding reagent Certificates of Analysis

TitleLot Number
Anti-poly-ADP-ribose binding reagent 3063342
Anti-poly-ADP-ribose binding reagent 2951394
Anti-poly-ADP-ribose binding reagent 2805278
Anti-poly-ADP-ribose binding reagent 3087164
Anti-poly-ADP-ribose binding reagent - 3208805 3208805
Anti-poly-ADP-ribose binding reagent - 3384383 3384383
Anti-poly-ADP-ribose binding reagent - 3510785 3510785
Anti-poly-ADP-ribose binding reagent - 3615429 3615429
Anti-poly-ADP-ribose binding reagent - 3736604 3736604
Anti-poly-ADP-ribose binding reagent - 3848523 3848523
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Technical Info

Title
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White Paper: Further considerations of antibody validation and usage.