Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABF1207 Anti-phospho-Syk (Tyr323)

This rabbit polyclonal Anti-phospho-Syk (Tyr323) antibody, Cat. No. ABF1207 is validated for use in Western Blotting for the detection of Syk Tyr323 phosphorylation.

Anti-phospho-Syk (Tyr323) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: SYK UniProt Number: P43405
ABF1207
100 µL  
Purchase on Sigma-Aldrich

Különleges ajánlatok

Áttekintés

Replacement Information

Különleges ajánlatok

100% Performance Guaranteed

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABF1207
Replaces07-915
DescriptionAnti-phospho-Syk (Tyr323)
Alternate Names
  • Tyrosine-protein kinase Syk, Tyr323 phosphorylated
  • p72-Syk, Tyr323 phosphorylated
  • p72Syk, Tyr323 phosphorylated
  • Spleen tyrosine kinase, Tyr323 phosphorylated
Background InformationTyrosine-protein kinase Syk (EC 2.7.10.2; UniProt P43405; also known as p72-Syk, p72Syk, Spleen tyrosine kinase) is encoded by the SYK gene (Gene ID 6850) in human. Spleen tyrosine kinase (Syk) is a non-receptor tyrosine kinase highly expressed by haematopoietic cells. Syk plays a crucial role not only in adaptive immune receptor signalling, but also in cellular adhesion, innate immune recognition, osteoclast maturation, platelet activation and vascular development. Syk adopts an autoinhibited conformation in its inactive state and is activated upon binding immunoreceptor tyrosine-based activation motifs (ITAMs) and phosphorylation of tyrosine residues in the two linker regions (a.a. 108-160 and 260-370) between SH1 (a.a. 15-107) and SH2 (a.a. 168-259), and between SH2 and the kinase domain (a.a. 371-631). Initial ITAM binding triggers rapid activation of Syk, whereas subsequent phosphorylation leads to prolonged activation. In additon, Syk can phosphorylate ITAMs and autophosphorylate its own link region tyrosines, providing a positive feedback loop following initial ITAM-mediated Syk activation. Syk also mediates signalling of receptors that do not contain an ITAM, including integrins and C-type lectins, via interaction with ITAM-containing adaptors, such as DAP12 (TYROBP) and the FcR γ-chain (FcRγ).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis rabbit polyclonal Anti-phospho-Syk (Tyr323) antibody, Cat. No. ABF1207 is validated for use in Western Blotting for the detection of Syk Tyr323 phosphorylation.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a sequence surrounding phosphorylated Tyr323 of human Syk.
EpitopepTyr323
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityTarget phosphotyrosine residue coresponds to pTyr323 of human Syk spliced isoform 1 and pTyr300 of human Syk spliced isoform 2. Sequence surrounding the target tyrosine is not well conserved in mouse and rat Syk.
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SYK
Modifications
  • Phosphorylation
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~72/69 kDa observed. 72.07/69.51 kDa (human Syk isoform 1/2) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Syk Tyr323 phosphorylation induction in 10 µg of lysate from hydrogen peroxide-treated Jurkat cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ABF1207 04054839069642

Documentation

Anti-phospho-Syk (Tyr323) MSDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-Syk (Tyr323) Certificates of Analysis

TitleLot Number
Anti-phospho-Syk (Tyr323) - 3492217 3492217
Anti-phospho-Syk (Tyr323) -2890159 2890159
Anti-phospho-Syk (Tyr323) -Q2753916 Q2753916
Anti-phospho-Syk (Tyr323) Polyclonal Antibody 2919543
Anti-phospho-Syk (Tyr323) Polyclonal Antibody 2955679
Anti-phospho-Syk (Tyr323) Polyclonal Antibody 2863774
Anti-phospho-Syk (Tyr323) Polyclonal Antibody_2837113 2837113
Anti-phospho-Syk (Tyr323), Polyclonal Antibody 2832824