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07-209-I Anti-phospho-SHC Antibody (Tyr239, Tyr240)

Detect SHC tyr239 and tyr240 phosphorylation using this rabbit polyclonal Anti-phospho-SHC (Tyr239, Tyr240) antiboody, Cat. No. 07-209-I, validated for use in Western Blotting.

Anti-phospho-SHC Antibody (Tyr239, Tyr240) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: SHC1, SHC, SHCA UniProt Number: P29353
07-209-I
100 μg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-209-I
DescriptionAnti-phospho-SHC Antibody (Tyr239, Tyr240)
Alternate Names
  • p46Shc (pTyr194/pTyr195)
  • p52Shc (pTyr239/pTyr240)
  • p66Shc (pTyr349/pTyr350)
  • SHC-transforming protein 1, tyrosine-phosphorylated
  • SH2 domain protein C1, tyrosine-phosphorylated
  • SHC-transforming protein 3, tyrosine-phosphorylated
  • SHC-transforming protein A, tyrosine-phosphorylated
  • Src homology 2 domain-containing-transforming protein C1, tyrosine-phosphorylated
Background InformationSHC-transforming protein 1 (UniProt P29353; also known as SH2 domain protein C1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1) is encoded by the SHC1 (also known as SHC, SHCA) gene (Gene ID 6464) in human. Originally identified as an SH2-containing proto-oncogene involved in growth factor signaling, Shc proteins function as adaptor molecules recruited by numerous receptors (e.g. integrins, GPCRs, and receptors for growth factors, antigens, cytokines, and hormones) for signling. In addition to the ubiquitously expressed Shc (a.k.a. ShcA by SHC1/SHCA gene), there exist also ShcB (by SHC2/SHCB gene) and ShcC (by SHC3/SHCC gene), which are predominantly expressed in neuronal cells. Alternative splicings result in six ShcA isoforms, including p66Shc, p52Shc, and p46Shc. All ShcA isoforms possess two distinct domains, PTB (a.a. 156-339) and SH2 (a.a. 488-579), that bind phosphotyrosine-containing sequences, as well as a central region (CH1; a.a. 340-487) that contains critical tyrosine phosphorylation sites. The phosphotyrosine-binding (PTB) domain is also referred to as phosphotyrosine-interaction domain or PID.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect SHC tyr239 and tyr240 phosphorylation using this rabbit polyclonal Anti-phospho-SHC (Tyr239, Tyr240) antiboody, Cat. No. 07-209-I, validated for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a target site sequence surrounding phosphorylated Tyr239 and Tyr240 of human spliced isoform p52Shc.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityNumbering of the target pTyr residues varies depending on the species and spliced isoforms. Target pTyr residues this rabbit polyclonal detects correspond to pTyr349/pTyr350 of human spliced isoform 6 and p66Shc, pTyr239/pTyr240 of human spliced isoform 7 and p52Shc, pTyr194/pTyr195 of isoform p46Shc, and pTyr135/pTyr136 of isoform 5 reported by UniProt (P29353).
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine, Chicken, Mouse, Rat, Rhesus Macaque based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SHC1
  • SHC
  • SHCA
Modifications
  • Phosphorylation
  • Phosphorylation
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~52 kDa observed. 62.82/51.61/46.67/40.42/62.89/51.68 kDa (human isoform p66Shc/p52Shc/p46Shc/5/6/7), 62.61/51.41/46.47 kDa (mouse isoform p66Shc/p52Shc/p47Shc), and 51.50 kDa (rat) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected SHC tyrosine phosphorylation induction in 10 µg of lysate from EGF-stimulated HEK293 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
07-209-I 04054839075773