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ABT58 Anti-phospho PKA-RII (Ser96) Antibody

This Anti-phospho PKA-RII (Ser96) Antibody is validated for use in WB for the detection of phospho PKA-RII (Ser96).

Anti-phospho PKA-RII (Ser96) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure, Signaling Research Sub-Category: Cytoskeletal Signaling, Kinases & Phosphatases Gene Symbol: PRKAR2A, PKR2, PRKAR2 UniProt Number: P12367
ABT58
100 µL  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT58
Replaces06-704
DescriptionAnti-phospho PKA-RII (Ser96) Antibody
Alternate Names
  • PRKAR2A
  • protein kinase
  • cAMP-dependent, regulatory, type II, alpha
Background InformationcAMP-dependent protein kinase type II-alpha regulatory subunit (PKA-RIIα) is an enzymatic protein belonging to the cAMP-dependent kinase regulatory chain family, a group of regulatory proteins involved in the PKA signaling pathway. This pathway has been shown to have regulatory roles in development, growth, and mortality of various organisms. PKA-RIIα is a subunit of the PKA type II regulatory chain, one of two PKA isoforms, and is involved in the regulation of membrane interactions through its binding to anchoring proteins, such as MAP2 kinase. Before its activation with cAMP, the enzyme is comprised of two catalytic chains and two regulatory chains, which split into two catalytic monomers and one regulatory dimmer after interacting with cAMP.
References
Product Information
FormatAffinity Purified
Control
  • Forskolin untreated and treated PC12 cell lysates
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho PKA-RII (Ser96) Antibody is validated for use in WB for the detection of phospho PKA-RII (Ser96).
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to mouse PKA-RIIalpha phosphorylated at Ser96.
EpitopePhosphorylated Ser96 of PKA-RIIalpha
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThe antibody recognizes PKA-RIIalpha when phosphorylated at Ser96.
Species Reactivity
  • Human
  • Rat
  • Mouse
Species Reactivity NoteDemonstrated to react with human and rat. Predicted to react with mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PRKAR2A
  • PKR2
  • PRKAR2
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

SUBUNIT STRUCTURE: The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4. Interacts with CBFA2T3 (by similarity).

TISSUE SPECIFICTY: Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

PTM: Phosphorylated by the activated catalytic chain.

SEQUENCE SIMILARITIES: Belongs to the cAMP-dependent kinase regulatory chain family.

Contains 2 cyclic nucleotide-binding domains.
Molecular Weight~ 51 kDa MW observed. The specificity of the target was shown by Lambda phosphatase treatment.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in Forskolin untreated and treated PC12 cell lysates.

Western Blot Analysis: 1 µg/mL of the antibody detected PKA-RIIalpha (Ser96) in 10 µg of Forskolin untreated and treated PC12 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ABT58 04053252746673

Documentation

Anti-phospho PKA-RII (Ser96) Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-phospho PKA-RII (Ser96) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho PKA-RII (Ser96) - 2387854 2387854
Anti-phospho PKA-RII (Ser96) - 2445071 2445071
Anti-phospho PKA-RII (Ser96) - 2325129 2325129
Anti-phospho PKA-RII (Ser96) - 3167641 3167641
Anti-phospho PKA-RII (Ser96) - NRG1774097 NRG1774097
Anti-phospho PKA-RII (Ser96) -2575529 2575529
Anti-phospho PKA-RII (Ser96) -2606901 2606901
Anti-phospho PKA-RII (Ser96) -2657462 2657462
Anti-phospho PKA-RII (Ser96) -2730801 2730801
Anti-phospho PKA-RII (Ser96) -2739022 2739022
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