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ABS194 Anti-phospho PDHE1-A type I (Ser300) Antibody

This Anti-phospho PDHE1-A type I (Ser300) Antibody is validated for use in WB, IC for the detection of phospho PDHE1-A type I (Ser300).

Anti-phospho PDHE1-A type I (Ser300) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: PDHA1, PHE1A UniProt Number: P08559
ABS194
50 µg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, B, XnWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS194
DescriptionAnti-phospho PDHE1-A type I (Ser300) Antibody
Alternate Names
  • Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
  • PDHE1-A type I
Background InformationIn many organisms, the pyruvate dehydrogenase complex catalyzes the overall, irreversible conversion of pyruvate to acetyl-CoA and CO2 in the aerobic, energy-generating pathways, in addition to serving as a key regulator for cardiac substrate selection. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). PDH is regulated by both pyruvate dehydrogenase kinase (PDK)-mediated phosphorylation and feedback inhibition.
References
Product Information
FormatAffinity Purified
Control
  • Dichloroacetate untreated and treated HEK 293 cell lysates
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho PDHE1-A type I (Ser300) Antibody is validated for use in WB, IC for the detection of phospho PDHE1-A type I (Ser300).
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PDHE1-A type I in dichloroacetate untreated and treated HEK 293 cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human PDHE1-A type I phosphorylated at Ser300.
EpitopePhosphorylated Ser300
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes PDHE1-A type I phosphorylated at Ser300.
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Bovine
  • Xenopus
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, Zebra Fish, and Xenopus based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • PDHA1
  • PHE1A
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

CATALYTIC ACTIVITY: Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

COFACTOR: Thiamine pyrophosphate.

ENZYME REGULATION: E1 activity is regulated by phosphorylation (inactivation) and dephosphorylation (activation) of the alpha subunit.

SUBUNIT STRUCTURE: Tetramer of 2 alpha and 2 beta subunits.

SUBCELLULAR LOCATION: Mitochondrion matrix.

TISSUE SPECIFICITY: Ubiquitous.
Molecular Weight~43 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in dichloroacetate untreated and treated HEK 293 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected PDHE1-A type I on 10 µg of dichloroacetate untreated and treated HEK 293 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ABS194 04053252633966

Documentation

Anti-phospho PDHE1-A type I (Ser300) Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-phospho PDHE1-A type I (Ser300) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho PDHE1-A type I (Ser300) 2480941
Anti-phospho PDHE1-A type I (Ser300) - 2356330 2356330
Anti-phospho PDHE1-A type I (Ser300) - 2388993 2388993
Anti-phospho PDHE1-A type I (Ser300) - 2397162 2397162
Anti-phospho PDHE1-A type I (Ser300) - 2445031 2445031
Anti-phospho PDHE1-A type I (Ser300) - 2271218 2271218
Anti-phospho PDHE1-A type I (Ser300) - 2327007 2327007
Anti-phospho PDHE1-A type I (Ser300) - 3216495 3216495
Anti-phospho PDHE1-A type I (Ser300) - 3690731 3690731
Anti-phospho PDHE1-A type I (Ser300) - 3807967 3807967
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