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ABS1515 Anti-phospho NFkB p52, (Ser222) Antibody

Anti-phospho NFkB p52, (Ser222) is an antibody against phospho NFkB p52 for use in Western Blotting.

Anti-phospho NFkB p52, (Ser222) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: NFKB2, LYT10 UniProt Number: Q00653
ABS1515
100 µL  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS1515
DescriptionAnti-phospho NFkB p52, (Ser222) Antibody
Alternate Names
  • Nuclear factor NF-kappa-B p100 subunit
  • DNA-binding factor KBF2
  • H2TF1
  • Lymphocyte translocation chromosome 10 protein
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2
  • Oncogene Lyt-10
  • Lyt10
  • Nuclear factor NF-kappa-B p52 subunit
Background InformationThe p52 NF-kB subunit is induced through activation of the alternative (noncanonical) NF-kB pathway in response to a range of stimuli such as lymphotoxin-b, BAFF and CD40, as well as DNA damage. The p52 formation results from proteolytic processing of its precursor protein p100 as a consequence of IKKalpha-mediated phosphorylation. The p52 is most commonly found as a heterodimer with RelB, although other dimer combinations have been identified by ChIP analysis. Ser222 of NF-kappa B p52 is phosphorylated by the kinase GSK3beta. Phosphorylation at S222 appears to regulate not only p52 partner dimerization but also the p52 dependent activation or repression of Skp2 gene expression.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho NFkB p52, (Ser222) is an antibody against phospho NFkB p52 for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected phospho NFkB p52, (Ser222) in CD40L and NTL cell lysate (Neil D. Perkins, Newcastle University).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the RHD domain of human phospho NFkB p52, (Ser222).
EpitopeRHD domain
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse, Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NFKB2
  • LYT10
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~52 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in cell lysate U2OS transfected with p52 WT.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho NFkB p52, (Ser222) in 10 µg of cell lysate U2OS transfected with p52 WT.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ABS1515 04055977127348