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06-1423 Anti-phospho-Lck (Tyr505) Antibody

Anti-phospho-Lck (Tyr505) Antibody detects level of phospho-Lck (Tyr505) & has been published & validated for use in WB.

Anti-phospho-Lck (Tyr505) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: LCK, lck UniProt Number: P06239
06-1423
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, M, Po, Sh, BWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number06-1423
DescriptionAnti-phospho-Lck (Tyr505) Antibody
Alternate Names
  • Tyrosine-protein kinase Lck
  • Leukocyte C-terminal Src kinase
  • LSK
  • Lymphocyte cell-specific protein-tyrosine kinase
  • Protein YT16
  • Proto-oncogene Lck
  • T cell-specific protein-tyrosine kinase
  • p56-LCK
Background InformationAnti-Phospho-Lck (Tyr505) detects endogenous levels of Lck only when phosphorylated at Tyr505. Lck is a member of the Src family of tyrosine kinases and is most commonly found in T cells. It associates with the cytoplasmic tails of the CD4 on T helper cells and CD8 co-receptors on cytotoxic T cells. The tyrosine phosphorylation cascade initiated by Lck leads to the intracellular mobilization of a calcium (Ca2+) ions and activation of important signaling cascades within the lymphocyte including the Ras-MEK-ERK pathway, which goes on to activate certain transcription factors such as NFAT, NF-κB, and AP-1. These transcription factors regulate the production of gene products, such as Interleukin-2.
References
Product Information
FormatAffinity Purified
Control
  • Hydrogen peroxide untreated and treated Jurkat cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-phospho-Lck (Tyr505) Antibody detects level of phospho-Lck (Tyr505) & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Lck phosphorylated at Tyr505.
EpitopePhosphorylated Tyr505
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes Lck phosphorylated at Tyr505.
Species Reactivity
  • Human
  • Rat
  • Mouse
  • Pig
  • Sheep
  • Bovine
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Rat, Mouse, Porcine, Sheep, and Bovine based on 100% sequence homology. Other homologies: Xenopus, Zebrafish, and Chicken (90% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described.
Gene Symbol
  • LCK
  • lck
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates RUNX3.

CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

ENZYME REGULATION: Inhibited by tyrosine phosphorylation.

SUBUNIT STRUCTURE: Binds to the cytoplasmic domain of cell surface receptors, such as CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB and to other protein kinases including CDK1, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with SQSTM1. Interacts with phosphorylated LIME1. Interacts with CBLB and PTPRH. Interacts with RUNX3.

SUBCELLULAR LOCATION: Cytoplasm. Cell membrane; Lipid-anchor; Cytoplasmic side. Note: Present in lipid rafts in an unactive form.

TISSUE SPECIFICITY: Expressed specifically in lymphoid cells.

DOMAIN: The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation.

PTM: Phosphorylated on Tyr-394, which increases enzymatic activity (By similarity). Phosphorylated on Tyr-505, which decreases activity. INVOLVEMENT IN DISEASE: Note=A chromosomal aberration involving LCK is found in leukemias. Translocation t(1;7)(p34;q34) with TCRB.

SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Contains 1 protein kinase domain.

Contains 1 SH2 domain.

Contains 1 SH3 domain.

MASS SPECTROMETRY: Molecular mass is 57869.42 Da from positions 2 - 509. Determined by MALDI.

SEQUENCE CAUTION: The sequence CAI22320.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence CAI22321.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Molecular Weight~56 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in hydrogen peroxide untreated and treated Jurkat cell lysate.

Western Blot Analysis: A 1:20,000 dilution of this antibody detected Lck on 10 µg of hydrogen peroxide untreated and treated Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
06-1423 04053252297137