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MABE943 Anti-phospho-Histone H3.3 (Ser31) Antibody, clone 1A8G10

Anti-phospho-Histone H3.3 Antibody, clone 1A8G10 is an antibody against phospho-Histone H3.3 for use in Western Blotting, ELISA.

Anti-phospho-Histone H3.3 (Ser31) Antibody, clone 1A8G10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: H3F3A, H3.3A, H3F3, H3F3B, H3.3B
 UniProt Number: P84243
MABE943
100 µg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ELISAMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE943
DescriptionAnti-phospho-Histone H3.3 (Ser31) Antibody, clone 1A8G10
Alternate Names
  • Histone H3.3 (Ser31)
  • Histone H3.3
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci. Cat. No. MABE943, Anti-phospho-Histone H3.3 (Ser31), clone 1A8G10 detects Histone H3.3 phosphorylated on Ser31. Sequence surrounding the target H3.3 phosphorylattion site is conserved in H3.3 of human, murine, and rat species (UniProt P84243, P84244, and P84245, respectively), but not in H3.1 or H3.2.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-phospho-Histone H3.3 Antibody, clone 1A8G10 is an antibody against phospho-Histone H3.3 for use in Western Blotting, ELISA.
Key Applications
  • Western Blotting
  • ELISA
Application NotesWestern Blotting Analysis: A representative lot detected phospho-Histone H3.3 (Ser31) in nocodazole treated HeLa cell lysate (Yoshimi, T., et al. (2013). Monoclon. Antib. Immunodiagn. Immunother. 32(2):119-124).
ELISA Analysis: A representative lot detected phospho-Histone H3.3 (Ser31) peptides (Yoshimi, T., et al. (2013). Mono. Anti. In Immunodiagnosis and Immunotherapy. 32(2):119-124).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human phospho-Histone H3.3 (Ser31).
EpitopeSurrounding H3.3 pSer31
Clone1A8G10
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Rat, Mouse based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • H3F3A
  • H3.3A
  • H3F3
  • H3F3B
  • H3.3B
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~17 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in nocodazole treated Hela cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected phospho-Histone H3.3 (Ser31) in 10 µg of nocodazole treated Hela cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABE943 04055977164145

Documentation

Anti-phospho-Histone H3.3 (Ser31) Antibody, clone 1A8G10 Certificates of Analysis

TitleLot Number
Anti-phospho-Histone H3.3 (Ser31), -Q2564185 Q2564185
Anti-phospho-Histone H3.3 (Ser31), clone 1A8G10 - 3328851 3328851

References

Reference overviewPub Med ID
A panel of specific monoclonal antibodies directed against various phosphorylated histones H3.
Yoshimi, T; Ohkawa, Y; Azuma, M; Tachibana, T
Monoclonal antibodies in immunodiagnosis and immunotherapy  32  119-24  2013

Kivonat megmutatása 		23607347 23607347

Brochure

Title
NPI Flyer- Epigenetics and Nuclear Function Feature

Technical Info

Title
White Paper: Further considerations of antibody validation and usage.