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ABE566 Anti-phospho Histone H3 (Tyr41) Antibody| ABE566

This Anti-phospho Histone H3 (Tyr41) Antibody is validated for use in western blotting, ICC & dot blot for the detection of phospho Histone H3 (Tyr41).

Anti-phospho Histone H3 (Tyr41) Antibody| ABE566 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ UniProt Number: P68431
ABE566
100 µg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICC, DBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE566
DescriptionAnti-phospho Histone H3 (Tyr41) Antibody| ABE566
Alternate Names
  • Histone H3.1
  • Histone H3/a
  • Histone H3/b
  • Histone H3/c
  • Histone H3/d
  • Histone H3/f
  • Histone H3/h
  • Histone H3/i
  • Histone H3/j
  • Histone H3/k
  • Histone H3/l
Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho Histone H3 (Tyr41) Antibody is validated for use in western blotting, ICC & dot blot for the detection of phospho Histone H3 (Tyr41).
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Dot Blot
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho Histone H3 (Tyr41) in HeLa and NIH/3T3 cells.

DyLight® is a registered trademark of Thermo Fisher Scientific.

Dot Blot Analysis: 0.5 µg/mL from a representative lot detected phospho Histone H3 (Tyr41) in an Absurance® Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance® Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3 phosphorylated at Tyr41.
EpitopeNear N-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3FA
  • HIST1H3B
  • H3FL
  • HIST1H3C
  • H3FC
  • HIST1H3D
  • H3FB
  • HIST1H3E
  • H3FD
  • HIST1H3F
  • H3FI
  • HIST1H3G
  • H3FH
  • HIST1H3H
  • H3FK
  • HIST1H3I
  • H3FF
  • HIST1H3J
  • H3FJ
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~17 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in colcemid treated HeLa acid extract.

Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H3 (Tyr41) in 10 µg of colcemid treated HeLa acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ABE566 04053252961137

Documentation

Anti-phospho Histone H3 (Tyr41) Antibody| ABE566 MSDS

Title

Safety Data Sheet (SDS) 

Anti-phospho Histone H3 (Tyr41) Antibody| ABE566 Certificates of Analysis

TitleLot Number
Anti-phospho Histone H3 (Tyr41) - Q2388157 Q2388157
Anti-phospho Histone H3 (Tyr41) - 3583793 3583793
Anti-phospho Histone H3 (Tyr41) - 3782208 3782208
Anti-phospho Histone H3 (Tyr41) - 4107712 4107712
Anti-phospho Histone H3 (Tyr41) -2673177 2673177
Anti-phospho Histone H3 (Tyr41) -2826033 2826033
Anti-phospho Histone H3 (Tyr41) Polyclonal Antibody 3084619
Anti-phospho Histone H3 (Tyr41) Polyclonal Antibody 3147031