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MABE939 Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody

This Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody is validated for use in Western Blotting and Immunocytochemistry and ELISA for the detection of phospho Histone H3 (Ser10).

Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: H3F3A, H3.3A, H3F3 UniProt Number: P84243
MABE939
100 µg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICC, ELISARPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE939
Replaces04-1093
DescriptionAnti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody
Alternate Names
  • H3S10P
  • Histone H3 (phospho Ser10)
  • H3 histone, family 3A
  • H3.3A
  • H3 histone, family 3B
  • H3.3B
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3.3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody is validated for use in Western Blotting and Immunocytochemistry and ELISA for the detection of phospho Histone H3 (Ser10).
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • ELISA
Application NotesImmunocytochemistry Analysis: A 1:5000 dilution from a representative lot detected Histone H3 (Ser10) in HeLa cells (Prof. Taro Tachibana, Osaka City University).
ELISA Analysis: A representative lot detected Histone H3 (Ser10) using differently phosphorylated and unphosphorylated peptides unmodified H3, H3 S10ph, and H3 S28ph (Prof. Taro Tachibana, Osaka City University).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Histone H3 (Ser10).
Clone6G8B7
Concentration1 mg/mL
HostRat
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • H3F3A
  • H3.3A
  • H3F3
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~17 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Histone H3 (Ser10) in 10 µg of nocodazole treated HeLa cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABE939 04053252977657

Documentation

Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody Certificates of Analysis

TitleLot Number
Anti-phospho Histone H3 (Ser10), -2687870 2687870
Anti-phospho Histone H3 (Ser10), clone 6G8B7 - Q2433686 Q2433686
Anti-phospho Histone H3 (Ser10), clone 6G8B7 - 3234485 3234485
Anti-phospho Histone H3 (Ser10), clone 6G8B7 - 3398037 3398037
Anti-phospho Histone H3 (Ser10), clone 6G8B7 - 3485890 3485890
Anti-phospho Histone H3 (Ser10), clone 6G8B7 - 3532435 3532435
Anti-phospho Histone H3 (Ser10), clone 6G8B7 - 3792974 3792974
Anti-phospho Histone H3 (Ser10), clone 6G8B7 - 3925334 3925334
Anti-phospho Histone H3 (Ser10), clone 6G8B7 - 3925343 3925343
Anti-phospho Histone H3 (Ser10), clone 6G8B7 - 4026459 4026459
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