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MABC1166 Anti-iASPP Antibody, clone LX128.5

Anti-iASPP Antibody, clone LX128.5 is an antibody against iASPP for use in Immunohistochemistry (Paraffin).

Anti-iASPP Antibody, clone LX128.5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: PPP1R13L, IASPP, NKIP1, PPP1R13BL, RAI UniProt Number: Q8WUF5
MABC1166
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Species ReactivityKey ApplicationsHostFormatAntibody Type
MIH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1166
DescriptionAnti-iASPP Antibody, clone LX128.5
Alternate Names
  • RelA-associated inhibitor
  • Inhibitor of apoptosis-stimulating protein of p53
  • Inhibitor of ASPP protein
  • NF-kB-interacting protein 1
  • PPP1R13B-like protein
  • Protein iASPP
Background InformationRelA-associated inhibitor (UniProt Q8WUF5; also known as Inhibitor of apoptosis-stimulating protein of p53, Inhibitor of ASPP protein, NF-kB-interacting protein 1, PPP1R13B-like protein, Protein iASPP) is encoded by the PPP1R13L (also known as IASPP, NKIP1, PPP1R13BL, RAI) gene (Gene ID 10848) in human. Inhibitor of apoptosis-stimulating protein of p53 (iASPP) belongs to the ASPP family of proteins (ASPP1, ASPP2, and iASPP) characterized by their ankyrin repeats (a.a. 659-691 & 692-724 in iASPP), SH3 domain (a.a. 758-820 in iASPP), and proline-rich (a.a. 54-602 in iASPP) sequences. iASPP is expressed at intercalated discs in post-mitotic cardiomyocytes, where it interacts with desmoplakin and desmin in cardiomyocytes to help maintain the integrity of desmosomes and intermediate filament networks. Desmosomes are anchoring junctions found in cells that endure physical stress such as cardiac myocytes or skin keratinocytes. Mutations of genes coding desmosome components, including IASPP/PPP1R13L, are found in human patients suffering from cardiomyopathy and in animal models of cardiomyopathy. iASPP deficiency caused by spontaneous mutation has been reported to be linked to a lethal autosomal recessive cardiomyopathy in Poll Hereford calves and Wa3 mice. iASSP-deficient mice with exon 8 deletion display features of arrhythmogenic right ventricular cardiomyopathy (ARVC) and die of sudden cardiac death.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-iASPP Antibody, clone LX128.5 is an antibody against iASPP for use in Immunohistochemistry (Paraffin).
Key Applications
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A representative lot immunostained intercalated discs in myocardial sections from adult wild-type, but not iASPP-deficient (iASPPΔ8/Δ8) mice (Notari, M., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(9):E973-E981).
Note: iASPP is expressed at intercalated discs. To prepare heart tissue sections for iASPP staining, it is important to cut the tissues at an angle parallel to the plane of the intercalated discs.
Biological Information
ImmunogenSynthetic linear peptide corresponding to a sequence from the N-terminal region of human iASPP.
EpitopeN-terminal region.
CloneLX128.5
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone LX128.5 immunostained intercalated discs in myocardial sections from wild-type, but not iASPP-deficient (iASPPΔ8/Δ8) adult mice (Notari, M., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(9):E973-E981).
IsotypeIgG1κ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Human based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PPP1R13L
  • IASPP
  • NKIP1
  • PPP1R13BL
  • RAI
Purification MethodProtein G Purified
UniProt Number
Molecular Weight89.09 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in mouse heart tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected iASPP in mouse heart tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABC1166 04055977334265