Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABS2194-100UG Anti-gp91phox (NOX2) Antibody, clone CL5

Anti-gp91phox (NOX2), clone CL5, Cat. No. MABS2194, is a mouse monoclonal antibody that detects Cytochrome b-245 heavy chain (gp91phox) and is tested for use in Western Blotting.

MABS2194-100UG
100 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberMABS2194-100UG
DescriptionAnti-gp91phox (NOX2) Antibody, clone CL5
Alternate Names
  • Cytochrome b-245 heavy chain
  • CGD91-phox
  • Cytochrome b(558) subunit beta
  • Cytochrome b558 subunit beta
  • Heme-binding membrane glycoprotein gp91phox
  • NADPH oxidase 2
  • Neutrophil cytochrome b 91 kDa polypeptide
  • Superoxide-generating NADPH oxidase heavy chain subunit
  • gp91-1
  • p22 phagocyte B-cytochrome
Background InformationCytochrome b-245 heavy chain (UniProt: P04839; also known as CGD91-phox, Cytochrome b(558) subunit beta, Cytochrome b558 subunit beta, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-generating NADPH oxidase heavy chain subunit, gp91-1, gp91-phox, p22 phagocyte B-cytochrome) is encoded by the CYBB (also known as NOX2) gene (Gene ID: 1536) in Human. Gp91phox is a multi-pass membrane glycoprotein of the NADPH oxidase family that contains three extracellular domains, six transmembrane domains, and four cytoplasmic domains. It contains four iron binding sites and has a ferric oxidoreductase domain that is localized in the amino acids 54-286. It is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide and plays an important role in the host defense function of phagocytic cells. It also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes. It can undergo polyubiquitination at lysine 48 by RNF145 that triggers its endoplasmic reticulum-associated degradation. Clone CL5 recognizes 13 amino acid sequence from the N-terminal region and displays reactivity with native and recombinant NOX2. Mutations in CYBB gene are known to cause granulomatous disease that is characterized by the inability of neutrophils and phagocytes to kill ingested life-threatening bacteria and fungus. (Ref.: (Ref.: Burritt, JB., et al. (2003). J. Immunol. 170 (12); 6082-6089; Baniulis, D., et al. (2005). Eur. J. Haematol. 74(4):337-347).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-gp91phox (NOX2), clone CL5, Cat. No. MABS2194, is a mouse monoclonal antibody that detects Cytochrome b-245 heavy chain (gp91phox) and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected gp91phox (NOX2) in Western Blotting applications (Baniulis, D., et. al. (2005). Eur J Haematol. 74(4):337-47; Baniulis, D., et. al. (2005). Biochim Biophys Acta. 1752(2):186-96).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenPartially purified human neutrophil flavocytochrome b588.
EpitopeN-terminal half
CloneCL5
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone CL5 is a mouse monoclonal antibody that detects human gp91phox/NOX2. It targets an epitope with in 13 amino acids from the N-terminal, third extracellular domain.
IsotypeIgG1
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CYBB
  • NOX2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~91 kDa observed; 65.34 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Human PMN cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected gp91phox (NOX2) in Human PMN cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABS2194-100UG 04065265872591