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07-1843 Anti-dimethyl (Lys4) dimethyl (Lys9) Histone H3 Antibody

Anti-dimethyl (Lys4) dimethyl (Lys9) Histone H3 Antibody is a rabbit polyclonal antibody for detection of dimethyl (Lys4) dimethyl (Lys9) Histone H3 also known as H3K4me2K9me2 has been validated in ChIP, WB, Mplex.

Anti-dimethyl (Lys4) dimethyl (Lys9) Histone H3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: H3.3A , H3.3B, H3F3 UniProt Number: P84243
07-1843
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, VrtWB, Mplex, ChIPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1843
DescriptionAnti-dimethyl (Lys4) dimethyl (Lys9) Histone H3 Antibody
Alternate Names
  • H3K4me2K9me2
  • Histone H3 (di methyl K4, di methyl K9)
  • H3 histone, family 3A
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.
References
Product Information
FormatAffinity Purified
Control
  • HeLa nuclear extract
PresentationAffinity purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-dimethyl (Lys4) dimethyl (Lys9) Histone H3 Antibody is a rabbit polyclonal antibody for detection of dimethyl (Lys4) dimethyl (Lys9) Histone H3 also known as H3K4me2K9me2 has been validated in ChIP, WB, Mplex.
Key Applications
  • Western Blotting
  • Multiplexing
  • Chromatin Immunoprecipitation (ChIP)
Application NotesChromatin Immunoprecipitation:
An independent lab has shown that this antibody performs in chromatin immunoprecipitation (ChIP).
Biological Information
ImmunogenSynthetic peptide corresponding to residues surrounding and including dimethlyated Lys 4 and dimethlyated Lys9 of Histone H3.
EpitopeDimethylated Lys4 & Lys9
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody only detects Histone H3 when dimethylated on both Lysine 4 and Lys9.
Species Reactivity
  • Human
  • Vertebrates
Species Reactivity NoteHuman tested. Expected to react with most other species based on extreme conserved homology among species.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Gene Symbol
  • H3.3A
  • H3.3B
  • H3F3
Modifications
  • Methylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION:Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Ref.14 Ref.18 Ref.22

SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into nucleosomes. Ref.14

SUBCELLULAR LOCATION: Nucleus.

DEVELOPMENTAL STAGE: Expressed throughout the cell cycle independently of DNA synthesis.

PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18.

Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription.

Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression.

Specifically enriched in modifications associated with active chromatin such as methylation at Lys-5, Lys-37 and Lys-80. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28, which are linked to gene repression, are underrepresented. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin.

Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase, probably DAPK3. Phosphorylation at 'Ser-11' by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation on Ser-32 is specific to regions bordering centromeres in metaphase chromosomes.

Ubiquitinated By similarity.

SEQUENCE SIMILARITY: Belongs to the histone H3 family.

SEQUENCE CAUTION:The sequence CAH73371.1 differs from that shown. Reason: Erroneous gene model prediction.

Molecular Weight~17 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting on HeLa acid extracted nuclear preps (positive) and recombinant Histone H3 (negative).

Western Blotting Analysis:
1:500 dilution of this antibody was used to dimethyl (Lys4) dimethyl (Lys9) Histone H3 in acid extracted HeLa nuclear extracts.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
07-1843 04053252320521

Documentation

Anti-dimethyl (Lys4) dimethyl (Lys9) Histone H3 Antibody Certificates of Analysis

TitleLot Number
Anti-dimethyl (Lys4) dimethyl (Lys9) Histone H3 - 1969077 1969077
Anti-dimethyl (Lys4) dimethyl (Lys9) Histone H3 - NG1607965 NG1607965
Anti-dimethyl (Lys4) dimethyl (Lys9) Histone H3 - NG1726956 NG1726956

References

Reference overviewPub Med ID
A chromatin activity-based chemoproteomic approach reveals a transcriptional repressome for gene-specific silencing.
Liu, C; Yu, Y; Liu, F; Wei, X; Wrobel, JA; Gunawardena, HP; Zhou, L; Jin, J; Chen, X
Nature communications  5  5733  2014

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