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OP17L Anti-c-Fos (Ab-1) Mouse mAb (2G9C3)

This Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of c-Fos (Ab-1).

Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP17L
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

KatalógusszámCsomagolás Menny./csomag
OP17L-200UG Üvegpalack 200 μg
Description
OverviewRecognizes both the ~55 kDa c-Fos and ~62 kDa v-Fos proteins in HeLa cells. Does not cross-react with the ~39 kDa Jun protein.
Catalogue NumberOP17L
Brand Family Calbiochem®
References
ReferencesJensen, D.E., et al. 1991. Oncogene 6, 1219.
Reiss, M., et al. 1990. Cancer Res. 50, 6641.
RouscherIII, F.J., et al. 1988. Science 240, 1010.
Sassone-Corsi, P., et al. 1988. Cell 54, 553.
Giardina, S.L., et al. 1987. Anal Biochem. 161, 109.
Verma, I.M., et al. 1987. Cancer Res. 49, 29.
Verma, I.M., et al. 1987. Cell 51, 513.
Muller, R. 1986. Viochem. Biophys. Acta. 823, 207.
Verma, P. 1986. Trends Genet. 2, 93.
Greenberg, M., et al. 1984. Nature (London) 311, 433.
Product Information
FormLyophilized
FormulationLyophilized from a volatile buffer, 200 µg BSA.
Positive controlHeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Not Paraffin Sections
Application NotesImmunoblotting (2.5-5 µg/ml)
Immunocytochemistry (2.5-5 µg/ml)
Paraffin Sections (not recommended)
Application CommentsRecognizes both cellular and viral forms of fos. Does not react with the ~39 kDa jun protein. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (KVEQLSPEEEEKRRIRRERNKMAAA) corresponding to amino acids 128-152 of human c-Fos
ImmunogenHuman
Clone2G9C3
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute in sterile PBS, PH 7.4, or sterile Tris-saline (20 mM Tris containing 150 mM NaCl), pH 7.4, to a final concentration of 200 µg/ml. To achieve complete solubilization, reconstitute at (4°C) with 0.1% sodium azide or aliqout and freeze (-20°C) for long-term storage. Note: sodium azide is cytotoxic.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
OP17L-200UG 04055977208818

Documentation

Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) MSDS

Title

Safety Data Sheet (SDS) 

Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) Certificates of Analysis

TitleLot Number
OP17L

References

Hivatkozások áttekintése
Jensen, D.E., et al. 1991. Oncogene 6, 1219.
Reiss, M., et al. 1990. Cancer Res. 50, 6641.
RouscherIII, F.J., et al. 1988. Science 240, 1010.
Sassone-Corsi, P., et al. 1988. Cell 54, 553.
Giardina, S.L., et al. 1987. Anal Biochem. 161, 109.
Verma, I.M., et al. 1987. Cancer Res. 49, 29.
Verma, I.M., et al. 1987. Cell 51, 513.
Muller, R. 1986. Viochem. Biophys. Acta. 823, 207.
Verma, P. 1986. Trends Genet. 2, 93.
Greenberg, M., et al. 1984. Nature (London) 311, 433.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-October-2007 RFH
ApplicationImmunoblotting (2.5-5 µg/ml)
Immunocytochemistry (2.5-5 µg/ml)
Paraffin Sections (not recommended)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with AG.8653X mouse myeloma cells. Recognizes the ~55 kDa c-fos and the ~62 kDa v-fos proteins.
BackgroundThe proto-oncogene fos has been implicated in cell growth, differentiation, and development. Proto-oncogene fos is induced by a large number of stimuli ranging from mitogens, differentiation-specific agents, pharmacological agents, etc. Induction of the 62,000 dalton fos protein is rapid but transient. The fos protein is associated with a 39,000 dalton protein which has been identified as the protein product of the jun proto-oncogene (c-jun). The fos/jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.
HostMouse
Immunogen speciesHuman
Immunogena synthetic peptide (KVEQLSPEEEEKRRIRRERNKMAAA) corresponding to amino acids 128-152 of human c-Fos
Clone2G9C3
IsotypeIgG2a
Specieshuman, mouse, rat
Positive controlHeLa cells
FormLyophilized
FormulationLyophilized from a volatile buffer, 200 µg BSA.
PreservativeNone
CommentsRecognizes both cellular and viral forms of fos. Does not react with the ~39 kDa jun protein. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute in sterile PBS, PH 7.4, or sterile Tris-saline (20 mM Tris containing 150 mM NaCl), pH 7.4, to a final concentration of 200 µg/ml. To achieve complete solubilization, reconstitute at (4°C) with 0.1% sodium azide or aliqout and freeze (-20°C) for long-term storage. Note: sodium azide is cytotoxic.
Toxicity Standard Handling
ReferencesJensen, D.E., et al. 1991. Oncogene 6, 1219.
Reiss, M., et al. 1990. Cancer Res. 50, 6641.
RouscherIII, F.J., et al. 1988. Science 240, 1010.
Sassone-Corsi, P., et al. 1988. Cell 54, 553.
Giardina, S.L., et al. 1987. Anal Biochem. 161, 109.
Verma, I.M., et al. 1987. Cancer Res. 49, 29.
Verma, I.M., et al. 1987. Cell 51, 513.
Muller, R. 1986. Viochem. Biophys. Acta. 823, 207.
Verma, P. 1986. Trends Genet. 2, 93.
Greenberg, M., et al. 1984. Nature (London) 311, 433.