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OP121 Anti-c-ErbB4 (Ab-1) Mouse mAb (H4.77.16)

Anti-c-ErbB4 (Ab-1) Mouse mAb (H4.77.16) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Erythroblastosis Virus, Anti-HER4, Anti-ErbB4

OP121
  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~180-185 kDa c-ErbB4 protein in MDA-MB-453 and T47D cells. Does not cross-react with c-ErbB1, c-ErbB2/c-Neu, or c-ErbB3.
Catalogue NumberOP121
Brand Family Calbiochem®
SynonymsAnti-Erythroblastosis Virus, Anti-HER4, Anti-ErbB4
References
ReferencesChen, X., et al. 1996. J. Biol. Chem. 271, 7620.
Carraway, K.L., et al. 1994. J. Biol. Chem. 269, 14303.
Tzahar, E., et al. 1994. J. Biol. Chem. 269, 25226.
Plowman, G.D., et al. 1993. Nature 366, 473.
Product Information
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Positive controlMDA-MB-453 or T-47D cells
Preservative≤0.1% sodium azide
Applications
Application ReferencesOriginal Clone Chen, X., et al. 1996. J. Biol. Chem. 271, 7620.
Key Applications Flow Cytometry
Immunoprecipitation
Not Western Blot
Application NotesFlow Cytometry (see comments)
Immunoprecipitation (10 µl/mg protein lysate)
Immunoblotting (not recommended)
Application Commentsc-erbB4 exists as two alternatively-spliced isoforms. Does not cross react with c-erbB1 (EGFR), c-erbB2/HER-2 or c-erbB3/HER-3. This antibody does not interfere with the receptor-heregulin binding and is particularly well suited for immunoprecipitation of unoccupied as well as the occupied c-erbB4 receptor. It reacts with intact cells, stimulates tyrosine kinase activity and efficiently stimulates internalization of c-erbB4 (use antibody at 10 µg/ml). This antibody has also been shown to work for flow cytometry. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of the extracellular domain of human c-erbB4
ImmunogenHuman
CloneH4.77.16
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
OP121 0

Documentation

Anti-c-ErbB4 (Ab-1) Mouse mAb (H4.77.16) MSDS

Title

Safety Data Sheet (SDS) 

Anti-c-ErbB4 (Ab-1) Mouse mAb (H4.77.16) Certificates of Analysis

TitleLot Number
OP121

References

Hivatkozások áttekintése
Chen, X., et al. 1996. J. Biol. Chem. 271, 7620.
Carraway, K.L., et al. 1994. J. Biol. Chem. 269, 14303.
Tzahar, E., et al. 1994. J. Biol. Chem. 269, 25226.
Plowman, G.D., et al. 1993. Nature 366, 473.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-October-2007 RFH
SynonymsAnti-Erythroblastosis Virus, Anti-HER4, Anti-ErbB4
ApplicationFlow Cytometry (see comments)
Immunoprecipitation (10 µl/mg protein lysate)
Immunoblotting (not recommended)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma NSO cells (see application references. Recognizes the ~180-185 kDa erbB4/HER-4 protein.
Backgroundc-erbB4/HER-4 is the fourth member of the type I transmembrane tyrosine kinase family of growth factor receptors that includes c-erbB1 (EGFR), an orphan receptor, c-erbB2 and c-erbB3, a receptor for the neu differentiation factor (NDF/heregulin). Like c-erbB3, c-erbB4 is also a receptor for NDF/heregulin. Each receptor member is characterized by ectodomains with two cysteine rich regions however, despite their structural homology, they differ in ligand specificities. c-erbB1/Her-1 binds EGF whereas c-erbB3 and c-erbB4 are the respective low and high affinity receptors for many isoforms of NDF/heregulin. Currently the ligand specificity for c-erbB2 has not been well characterized and thus remains the 'orphan receptor'. All four receptor members are expressed in breast cancer presumably a result of gene amplification. Overexpression of perhaps all of the receptors can also occur, probably as a result of increased mRNA transcription.
HostMouse
Immunogen speciesHuman
Immunogena recombinant protein consisting of the extracellular domain of human c-erbB4
CloneH4.77.16
IsotypeIgG₁
Specieshuman, mouse, rat
Positive controlMDA-MB-453 or T-47D cells
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
Commentsc-erbB4 exists as two alternatively-spliced isoforms. Does not cross react with c-erbB1 (EGFR), c-erbB2/HER-2 or c-erbB3/HER-3. This antibody does not interfere with the receptor-heregulin binding and is particularly well suited for immunoprecipitation of unoccupied as well as the occupied c-erbB4 receptor. It reacts with intact cells, stimulates tyrosine kinase activity and efficiently stimulates internalization of c-erbB4 (use antibody at 10 µg/ml). This antibody has also been shown to work for flow cytometry. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Toxicity Standard Handling
ReferencesChen, X., et al. 1996. J. Biol. Chem. 271, 7620.
Carraway, K.L., et al. 1994. J. Biol. Chem. 269, 14303.
Tzahar, E., et al. 1994. J. Biol. Chem. 269, 25226.
Plowman, G.D., et al. 1993. Nature 366, 473.
Application referencesOriginal Clone Chen, X., et al. 1996. J. Biol. Chem. 271, 7620.