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MABE330 Anti-c-ErbB2/c-Neu Antibody, clone 3B5

Anti-c-ErbB2/c-Neu Antibody, clone 3B5 is a Mouse Monoclonal Antibody for detection of c-ErbB2/c-Neu also known as MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2 or CD340, has been validated in W

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Anti-c-ErbB2/c-Neu Antibody, clone 3B5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: ERBB2, HER2, MLN19, NEU, NGL UniProt Number: P04626
MABE330
100 µg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE330
Replaces04-1127
DescriptionAnti-c-ErbB2/c-Neu Antibody, clone 3B5
Alternate Names
  • Receptor tyrosine-protein kinase erbB-2
  • Metastatic lymph node gene 19 protein
  • MLN 19
  • Proto-oncogene Neu
  • Proto-oncogene c-ErbB-2
  • Tyrosine kinase-type cell surface receptor HER2
  • p185erbB2
  • CD340
Background InformationThe ErbB2 (HER2, c-Neu, ErbB2, c-erbB2, or HER2/neu) protein is member of the human epidermal growth factor receptor (HER) family. It is a receptor tyrosine kinase containing an intracellular kinase domain and an extracellular ligand domain, although no endogenous ligand has yet been found. ErbB2 dimerizes with ErbB1, 3, and 4, and can activate different pathways including the PI3K, PLCγ, and MAPK pathways, depending on the ErbB receptor involved. Coupling with ErbB3 activates the PI3K pathway, whereas coupling with any of these three receptors can induce the MAPK pathway. ErbB2 signaling promotes cell proliferation and inhibits apoptosis. ErbB2 is overexpressed in various cancers and may play a role in the development and metastasis of gliomas, and ovarian, breast, lung, and gastric cancer.
Product Information
FormatPurified
Control
  • SK-BR-3 cell lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-c-ErbB2/c-Neu Antibody, clone 3B5 is a Mouse Monoclonal Antibody for detection of c-ErbB2/c-Neu also known as MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2 or CD340, has been validated in WB & IHC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected c-ErbB2/c-Neu in human breast cancer tissue.
Biological Information
ImmunogenLinear peptide corresponding human c-ErbB2/c-Neu.
Clone3B5
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ERBB2
  • HER2
  • MLN19
  • NEU
  • NGL
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~185 kDa observed. Uniprot describes four isoforms at ~138 kDa (isoform 1), ~63 kDa (isoform 2), ~71 kDa (isoform 3), and ~137 kDa (isoform 4), which can be highly glycosylated and phosphorylated. This antibody may detect one or more isoforms. Uncharacterized bands may be observed at ~27 kDa and ~38 kDa in some cell lysates.
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in SK-BR-3 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected c-ErbB2/c-Neu in 10 µg of SK-BR-3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Katalógusszám GTIN
MABE330 04053252522239