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06-599-AF647 Anti-acetyl-Histone H3 Antibody, Alexa Fluor® 647 Conjugate

This Anti-acetyl-Histone H3 Antibody, Alexa Fluor® 647 Conjugate is validated for use in Immunocytochemistry for the detection of acetyl-Histone H3.

Anti-acetyl-Histone H3 Antibody, Alexa Fluor® 647 Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: HIST3H3, H3FT UniProt Number: Q16695
06-599-AF647
100 µL  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MICCRbAlexaFluor®647Polyclonal Antibody
Description
Catalogue Number06-599-AF647
DescriptionAnti-acetyl-Histone H3 Antibody, Alexa Fluor® 647 Conjugate
Alternate Names
  • H3 histone family, member T
  • Histone 3, H3
  • Histone cluster 3, H3
Background InformationHistone H3 is one of the five main histone proteins that are involved in the structure of chromatin in eukaryotic cells. The chromatin fiber is composed on repetitive units, known as nucleosomes, which are comprised of an octamer of core histones (two each of H2A, H2B, H3, and H4), around which DNA are wrapped. Histone H3 features a main globular domain and a long N-terminal tail. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 is specifically phosphorylated during both mitosis and meiosis in patterns that are specifically coordinated in space and time. These phosphorylations are initiated at different phases of the cell division. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
References
Product Information
FormatAlexaFluor®647
PresentationPurified rabbit antibody conjugate in buffer containing PBS, 15 mg/mL BSA and 0.1% Sodium Azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-acetyl-Histone H3 Antibody, Alexa Fluor® 647 Conjugate is validated for use in Immunocytochemistry for the detection of acetyl-Histone H3.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected acetyl-Histone H3 in NIH/3T3 cells.
Biological Information
ImmunogenSynthetic peptide corresponding to human histone H3 N-terminal sequence with acetylated Lys9 and Lys14
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityAntibody recognizes histone H3 with acetylated Lys9 and/or Lys14.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology. Other homologies: Broad species cross-reactivity expected based on sequence similarity.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST3H3
  • H3FT
Modifications
  • Acetylation
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight17 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected acetyl-Histone H3 in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
06-599-AF647 04055977182002

Documentation

Anti-acetyl-Histone H3 Antibody, Alexa Fluor® 647 Conjugate MSDS

Title

Safety Data Sheet (SDS) 

Anti-acetyl-Histone H3 Antibody, Alexa Fluor® 647 Conjugate Certificates of Analysis

TitleLot Number
Anti-acetyl-Histone H3, Alexa Fluor® -2800494 2800494
Anti-acetyl-Histone H3, Alexa Fluor® 647 Conjugate 2884279
Anti-acetyl-Histone H3, Alexa Fluor® 647 Conjugate 3040395
Anti-acetyl-Histone H3, Alexa Fluor® 647 Conjugate - 3738038 3738038
Anti-acetyl-Histone H3, Alexa Fluor® 647 Conjugate - 3887100 3887100
Anti-acetyl-Histone H3, Alexa Fluor® 647 Conjugate - 4162290 4162290
Anti-acetyl-Histone H3, Alexa Fluor® 647 Conjugate -2736368 2736368
Anti-acetyl-Histone H3, Alexa Fluor® 647 Conjugate -Q2586390 Q2586390

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.