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05-776 Anti-ZAP-70 Antibody, clone 1E7.2

Anti-ZAP-70 Antibody, clone 1E7.2 is an antibody against ZAP-70 for use in IC, IP & WB.

Anti-ZAP-70 Antibody, clone 1E7.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Immunological Signaling Gene Symbol: ZAP70, ZAP-70, TZK, STD, SRK UniProt Number: P43403
05-776
200 µg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MICC, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-776
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-ZAP-70 Antibody, clone 1E7.2
OverviewNote: This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California
References
Product Information
FormatPurified
Presentationof 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Quality LevelMQ100
Applications
ApplicationAnti-ZAP-70 Antibody, clone 1E7.2 is an antibody against ZAP-70 for use in IC, IP & WB.
Key Applications
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blotting
Biological Information
ImmunogenGST fusion protein between the second SH2 domain and precedes the kinase domain corresponding to residues 282-307 of human ZAP-70
Clone1E7.2
HostMouse
SpecificityZap-70
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Gene Symbol
  • ZAP70
  • ZAP-70
  • TZK
  • STD
  • SRK
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P43403 # Associates with the T-cell antigen receptor zeta chain (CD3Z). Plays a role in lymphocyte activation.
SIZE: 619 amino acids; 69872 Da
SUBUNIT: Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB (By similarity). Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 (By similarity).
TISSUE SPECIFICITY: Expressed in T- and natural killer cells.
DOMAIN: SwissProt: P43403 The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.
PTM: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
DISEASE:SwissProt: P43403 # Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. & Contains 1 protein kinase domain. & Contains 2 SH2 domains.
Molecular WeightMr ~70kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from Jurkat cells
Sales RestrictionsDue to license agreement restrictions, this product cannot be purchased for resale.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions2 years at -20°C
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
05-776 04053252624308

Documentation

Anti-ZAP-70 Antibody, clone 1E7.2 MSDS

Title

Safety Data Sheet (SDS) 

Anti-ZAP-70 Antibody, clone 1E7.2 Certificates of Analysis

TitleLot Number
Anti-ZAP-70, clone 1E7.2 - 26824 26824
Anti-ZAP-70, clone 1E7.2 - 3190950 3190950
Anti-ZAP-70, clone 1E7.2 - 3897311 3897311
Anti-ZAP-70, clone 1E7.2 - DAM1739159 DAM1739159
Anti-ZAP-70, clone 1E7.2 - JBC1363175 JBC1363175
Anti-Zap-70, clone 1E7.2 (mouse monoclonal IgG1) - 2464566 2464566
Anti-Zap-70, clone 1E7.2 (mouse monoclonal IgG1) - 2187218 2187218
Anti-Zap-70, clone 1E7.2 (mouse monoclonal IgG1) - 2273888 2273888
Anti-Zap-70, clone 1E7.2 -2521194 2521194

References

Reference overviewPub Med ID
TCR signaling: another Abl-bodied kinase joins the cascade.
Wange, Ronald L
Curr. Biol., 14: R562-4 (2004)  2004

Kivonat megmutatása 		15268875 15268875