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MABN791 Anti-VAP-B Antibody, clone 3C6.2

Anti-VAP-B, clone 3C6.2, Cat. No. MABN791, is a highly specific mouse monoclonal antibody that targets VAMP-associated protein B (VAP-B) and has been tested in Immunostochemistry (Paraffin) and Western Blotting applications.

Anti-VAP-B Antibody, clone 3C6.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: VAPB, ALS8, UNQ484/PRO983 UniProt Number: O95292
MABN791
100 μg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN791
DescriptionAnti-VAP-B Antibody, clone 3C6.2
Alternate Names
  • Vesicle-associated membrane protein-associated protein B
  • VAMP-associated protein B
  • VAMP-B
  • VAP-B
Background InformationVesicle-associated membrane protein-associated protein B (UniProt O95292; also known as VAMP-associated protein B, VAMP-B, VAP-B) is encoded by the VAPB (also known as ALS8, UNQ484/PRO983) gene (Gene ID 9217) in human. VAMP-associated proteins (VAPs) are type IV membrane proteins that are well conserved among species. There exist three human VAPs encoded by two genes, with VAPA encoding VAP-A and VAPB encoding VAP-B and VAP-C. VAPs generally localize at the endoplasmic reticulum (ER), although they are also reported to localize at other subcellular organelles in some species and cell types. VAPs were shown to have important roles in non-vesicular lipid transport, lipid metabolism, the regulation of ER structure, and the unfolded protein response through MSP domain-mediated interaction with FFAT motifs. VAP-B functions as an adaptor protein to recruit target proteins to the ER and execute various cellular functions, including lipid transport, membrane traffic, and ER stress response. VAP-B binds the catalytic subunit Rab3 GTPase activating protein 1 (Rab3GAP1), but not the non-catalytic subunit Rab3GAP2 of the heterodimeric Rab3GAP complex. VAP-B and Rab3GAP interaction is mediated through the Rab3GAP1 acidic tract (FFAT)-like motif and is essential for VAP-B-dependent regulation of the nuclear envelope protein transport through the ER-Golgi intermediate compartment (ERGIC). VAP-B contains a major sperm protein (MSP; a.a. 7-124) domain at the N-terminal cytoplasmic region, followed by a coiled-coil region (a.a. 2-222), and a transmembrane (TM) domain (a.a. 223-243).
References
Product Information
FormatPurified
PresentationPurified mouse IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-VAP-B, clone 3C6.2, Cat. No. MABN791, is a highly specific mouse monoclonal antibody that targets VAMP-associated protein B (VAP-B) and has been tested in Immunostochemistry (Paraffin) and Western Blotting applications.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected VAP-B in human brain and kidney tissue sections.
Biological Information
ImmunogenGST-tagged recombinant fragment from the extracellular coiled coil region of human VAP-B.
Clone3C6.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3C6.2 epitope lies within VAP-B extracellular coiled coil region not present in isoform VAP-C.
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • VAPB
  • ALS8
  • UNQ484/PRO983
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~29 kDa observed. 27.10 kDa calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected VAP-B in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABN791 04054839057250