Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABC1434 Anti-UVRAG-FS mutant

Anti-UVRAG-FS mutant, Cat. No. ABC1434, detects FS mutant UV radiation resistance-associated gene protein. Validated for use in Immunoprecipitation and Western Blotting.

Anti-UVRAG-FS mutant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: UVRAG UniProt Number: Q9P2Y5
ABC1434
100 µL  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPRbSemi-PurifiedPolyclonal Antibody
Description
Catalogue NumberABC1434
DescriptionAnti-UVRAG-FS mutant
Alternate Names
  • UV radiation resistance-associated gene protein
  • p63
  • UVRAG-FS
Background InformationUV radiation resistance-associated gene protein (UniProt: Q9P2Y5; also known as p63) is encoded by UVRAG gene (Gene ID: 7405) in human. UVRAG is a key autophagic tumor suppressor that is shown to be mutated in several common human cancers. UVRAG as a critical regulator of intracellular membrane trafficking, autophagy, and chromosomal stability. It contains four functional domains, a proline-rich domain, a lipid-binding C2 domain, a Beclin1-binding coiled-coil domain (CCD) and a C-terminal domain. UVRAG is truncated by a frameshift (FS) mutation in colorectal cancer cells. UVRAG-FS is shown to abolish the normal functions of UVRAG, including autophagy, in a dominant-negative manner. Ectopic expression of UVRAG-FS in both embryonic stem cells and cancer cells results in extensive centrosome amplification and concomitant aneuploidy. UVRAG-FS can also enhance the sensitivity to anticancer agents, such as 5-fluorouracil (5-FU), oxaliplatin, and irinotecan. Expression of UVRAG-FS is reported to trigger colorectal cancer metastatic spread through Rac1 activation and epithelial-to-mesenchymal transition, independently of autophagy. UVRAG-FS metastatic tumors display higher levels of p62 than primary tumors, which indicates suppressed autophagy. (Ref.: He, S., et al. (2015). Nat Commun. 6: 7839).
References
Product Information
FormatSemi-Purified
PresentationRabbit polyclonal antibody 1XPBS with 0.05% azide.
Quality LevelMQ100
Applications
ApplicationAnti-UVRAG-FS mutant, Cat. No. ABC1434, detects FS mutant UV radiation resistance-associated gene protein. Validated for use in Immunoprecipitation and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesWestern Blotting Analysis: A representative lot detected UV radiation resistance-associated gene protein in Wild-type (WT) and FS mutant UVRAG protein expression in MSS and MSI CRC cell lines (Data courtesy of Dr Liang Laboratory).

Western Blotting Analysis: A representative lot detected Wild-type (WT) and FS mutant UVRAG protein expression in MSS and MSI CRC cell lines. (He, S., et. al. (2015). Nat Commun. 6:7839).

Immunoprecipitation Analysis: A representative lot detected Wild-type (WT) and FS mutant UVRAG protein expression in MSS and MSI CRC cell lines. (He, S., et. al. (2015). Nat Commun. 6:7839).
Biological Information
ImmunogenA frameshift (FS)-UVRAG derived linear peptide.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody targets an internal sequence in the N-terminal half and detects UVRAG-FS by Western blotting.
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • UVRAG
Purification MethodAmmonium Sulfate
UniProt Number
Molecular Weight~32 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in WT/ Mutant UVRAG-FS lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UV radiation resistance-associated gene protein in Wild-type (WT) and FS mutant UVRAG protein expression in microsatellite stable (MSS) and microsatellite instable (MSI) colorectal cancer cell lines.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ABC1434 04054839156434