Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

CP07 Anti-β-Tubulin Mouse mAb (DM1B)

Anti-β-Tubulin Mouse mAb (DM1B) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CP07
  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~60 kDa tubulin protein found in all eukaryotic cells. May be used as a positive control.
Catalogue NumberCP07
Brand Family Calbiochem®
References
ReferencesBlose, S.H., et al. 1984. J. Cell. Biol. 98, 847.
Lin, J.J.C. 1984. in Monoclonal antibodies and functional cell lines (Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds.) Plenum Publishing Corp.
Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Positive controlAny cell line
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Application NotesImmunoblotting (5 µg/ml)
Immunofluorescence (5 µg/ml)
Application CommentsTubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. β-Tubulin has a molecular weight of ~60 kDa (i.e. ~57 kDa in gerbils). Use fresh lysates for immunoblotting, as tubulin may not be detected after a freeze/thaw cycle. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogennative chick brain microtubules
ImmunogenChicken
CloneDM1B
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
CP07 0

Documentation

Anti-β-Tubulin Mouse mAb (DM1B) MSDS

Title

Safety Data Sheet (SDS) 

Anti-β-Tubulin Mouse mAb (DM1B) Certificates of Analysis

TitleLot Number
CP07

References

Hivatkozások áttekintése
Blose, S.H., et al. 1984. J. Cell. Biol. 98, 847.
Lin, J.J.C. 1984. in Monoclonal antibodies and functional cell lines (Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds.) Plenum Publishing Corp.
Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-August-2007 RFH
ApplicationImmunoblotting (5 µg/ml)
Immunofluorescence (5 µg/ml)
DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c57 mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~60 kDa β-tubulin protein.
BackgroundCytoskeletal proteins are found in highly organized arrays within the cytoplasm of higher eukaryotic cells. These proteins are intimately involved in functions such as cell and intracellular organelle movement, maintenance of cell shape and endocytosis. The cytoskeleton of most eukaryotic cells is comprised of at least three fibrous systems each with accessory proteins: the microfilaments, composed of actin; the microfibrils, composed of tubulin; and the intermediate filaments composed of vimentin. Monoclonal antibodies have been used to study the components of the cytoskeleton and their intracellular location, changes in the expression of these elements during differentiation, and the function of the cytoskeleton in living cells by microinjection of the antibodies.
HostMouse
Immunogen speciesChicken
Immunogennative chick brain microtubules
CloneDM1B
IsotypeIgG₁
Specieschicken, gerbil, human, mouse, rat
Positive controlAny cell line
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsTubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. β-Tubulin has a molecular weight of ~60 kDa (i.e. ~57 kDa in gerbils). Use fresh lysates for immunoblotting, as tubulin may not be detected after a freeze/thaw cycle. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesBlose, S.H., et al. 1984. J. Cell. Biol. 98, 847.
Lin, J.J.C. 1984. in Monoclonal antibodies and functional cell lines (Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds.) Plenum Publishing Corp.
Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.