Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MAB13429 Anti-TIMP-1 Antibody, clone 102D1

MAB13429
100 µg  
Purchase on Sigma-Aldrich

Különleges ajánlatok

Áttekintés

Replacement Information

Különleges ajánlatok

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB13429
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-TIMP-1 Antibody, clone 102D1
Alternate Names
  • Tissue Inhibitor of Metalloproteinase-1
References
Product Information
FormatPurified
Control
  • POSITIVE CONTROL: Cytotrophoblastic columns, the endothelial and fibroblastic stromal cells of villi, and the large decidualized cells of decidual membrane in placenta1.
PresentationPurified from ascites fluid by Protein G chromatography. Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-TIMP-1 Antibody, clone 102D1 is an antibody against TIMP-1 for use in WB, IH(P).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting: 1-2 μg/mL for 2 hours at room temperature

Immunohistochemistry: Formalin-fixed, paraffin-embedded tissues: 1-2 μg/mL for 30 min. at room temperature.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenRecombinant human TIMP-1.
Clone102D1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityIt recognizes a glycoprotein of 28.5 kDa which is identified as tissue inhibitor of metalloproteinases-1 (TIMP-1). TIMP-1 and TIMP-2 have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72 kDa type IV collagenase (also known as MMP-2 or gelatinase A), and latent and active forms 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Cellular Localization:

Cytoplasmic
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.
Gene Symbol
  • TIMP1
  • TIMP
  • FLJ90373
  • CLGI
  • EPA
  • EPO
  • TIMP-1
  • HCI
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01033 # Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
SIZE: 207 amino acids; 23171 Da
SUBCELLULAR LOCATION: Secreted.
PTM: The activity of TIMP1 is dependent on the presence of disulfide bonds.
SIMILARITY: SwissProt: P01033 ## Belongs to the protease inhibitor I35 (TIMP) family. & Contains 1 NTR domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months after date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MAB13429 04053252610288

Documentation

Anti-TIMP-1 Antibody, clone 102D1 MSDS

Title

Safety Data Sheet (SDS) 

Anti-TIMP-1 Antibody, clone 102D1 Certificates of Analysis

TitleLot Number
MOUSE ANTI-HUMAN TIMP-1 (TISSUE INHIBITOR OF METALLOPROTEINASE-1) 3070785
MOUSE ANTI-HUMAN TIMP-1 (TISSUE INHIBITOR OF METALLOPROTEINASE-1) MONOCLONAL ANTIBODY - 2460959 2460959
MOUSE ANTI-HUMAN TIMP-1 (TISSUE INHIBITOR OF METALLOPROTEINASE-1) - 3715669 3715669
MOUSE ANTI-HUMAN TIMP-1 (TISSUE INHIBITOR OF METALLOPROTEINASE-1) - 3805855 3805855
MOUSE ANTI-HUMAN TIMP-1 (TISSUE INHIBITOR OF METALLOPROTEINASE-1) - 3851163 3851163
MOUSE ANTI-HUMAN TIMP-1 (TISSUE INHIBITOR OF METALLOPROTEINASE-1) - 3903782 3903782
MOUSE ANTI-HUMAN TIMP-1 (TISSUE INHIBITOR OF METALLOPROTEINASE-1) - 4008814 4008814
MOUSE ANTI-HUMAN TIMP-1 (TISSUE INHIBITOR OF METALLOPROTEINASE-1) -2508779 2508779
MOUSE ANTI-HUMAN TIMP-1 (TISSUE INHIBITOR OF METALLOPROTEINASE-1) -2722609 2722609
MOUSE ANTI-HUMAN TIMP-1 (TISSUE INHIBITOR OF METALLOPROTEINASE-1) MONOCLONAL ANTIBODY 2944441

References

Reference overviewSpeciesPub Med ID
Angiotensin-II and rosuvastatin influence matrix remodeling in human mesangial cells via metalloproteinase modulation.
Solini A, Rossi C, Santini E, Madec S, Salvati A, Ferrannini E.
Journal of hypertension  29  1930-9  2010

Kivonat megmutatása
21881526 21881526
Pattern of expression of inflammatory markers in adipose tissue of untreated hypertensive patients.
Stephanie Madec,Massimo Chiarugi,Eleonora Santini,Chiara Rossi,Paolo Miccoli,Ele Ferrannini,Anna Solini
Journal of hypertension  28  2009

Kivonat megmutatása
20589975 20589975
A disintegrin and metalloprotease 21 (ADAM21) is associated with neurogenesis and axonal growth in developing and adult rodent CNS.
Peng Yang, K Adam Baker, Theo Hagg
The Journal of comparative neurology  490  163-79  2004

Kivonat megmutatása
Rat16052496 16052496

Data Sheet

Title
Anti-TIMP-1, clone 102D1 - Data Sheet