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ABN1488 Anti-TIAM-1

Anti-TIAM-1 Antibody, Cat. No. ABN1488, is a highly specific rabbit polyclonal antibody, that targets TIAM-1 and has been tested in Western Blotting.

Anti-TIAM-1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: TIAM1 UniProt Number: Q13009
ABN1488
100 μg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
MWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN1488
DescriptionAnti-TIAM-1
Alternate Names
  • T-lymphoma invasion and metastasis-inducing protein 1
  • TIAM-1
Background InformationT-lymphoma invasion and metastasis-inducing protein 1 (UniProt Q13009; also known as TIAM-1) is encoded by the TIAM1 gene (Gene ID 7074) in human. TIAM-1 is a ubiquitously expressed guanine nucleotide exchange factor (GEF) that specifically activates the Rho-like GTPase Rac1 in response to growth factors and cell-substrate interaction. TIAM-1 associates with GTP-bound Ras and functions as an effector that mediates Rac activation by Ras. TIAM-1 promotes the formation of adherens junctions and the induction of an epithelial-like phenotype in mesenchymal cells, and is essential for the formation and maintenance of cadherin-based adhesions. Tiam1-knockdown in mice protects against Ras-induced skin carcinogenesis as a result of increased apoptosis and reduced proliferation/growth of keratinocytes. However, the fewer skin tumors developed progress more frequently to malignancy in Tiam1-knockout mice, indicating that loss of Tiam1 stimulates malignant conversion. Cellular TIAM-1 is targeted for proteasome-dependent degradation by the SCFbTrCP ubiquitin ligase in cooperation with casein kinase 1 and the MEK/ERK pathway upon mitogenic stimulation.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-TIAM-1 Antibody, Cat. No. ABN1488, is a highly specific rabbit polyclonal antibody, that targets TIAM-1 and has been tested in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a C-terminal region sequence of human TIAM-1.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody targets a C-terminal region of human and mouse TIAM-1 sequences reported by UniProt (Q13009 and Q60610).
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Human, Rhesus Macaque, Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • TIAM1
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~190 kDa observed. 177.4/170.4 kDa (human isoform 1/2) and 177.4 kDa (mouse) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Neuro-2a cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected TIAM-1 in 10 µg of Neuro-2a mouse neuroblastoma cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ABN1488 04054839049750