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SC1002 Anti-Sox2 Mouse mAb (245610)

This Anti-Sox2 Mouse mAb (245610) is validated for use in Immunoblotting, Immunocytochemistry, Flow Cytometry for the detection of Sox2.

Anti-Sox2 Mouse mAb (245610) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
SC1002
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Products

KatalógusszámCsomagolás Menny./csomag
SC1002-100UG Muanyagampulla 100 μg
Description
OverviewRecognizes the ~34 kDa Sox2 protein in NTERA-2 cells.
Catalogue NumberSC1002
Brand Family Calbiochem®
References
ReferencesAvilion, A.A., et al. 2003.Genes Dev. 17, 126.
Graham, V., et al. 2003.Neuron 39, 749.
Stevanovic, M. 2003.Mol. Biol. Rep. 30, 127.
Kishi, M., et al. 2000.Development 127, 791.
Uwanogho, D., et al. 1995.Mech. Dev. 49, 23.
Yuan, H., et al. 1995.Genes Dev. 9, 2635.
Product Information
FormLyophilized
FormulationLyophilized from 0.2 µm-filter sterilized PBS, 5% trehalose.
Positive controlNTERA-2 cells
Quality LevelMQ100
Applications
Key Applications Flow Cytometry
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesImmunoblotting (1-2 µg/ml)
Immunocytochemistry (10 µg/ml; see comments)
Flow Cytometry (see comments)
Application CommentsFor immunocytochemistry, cells should be fixed in PBS containing 4% paraformaldehyde for 20 min, followed by blocking in PBS/10% normal donkey serum/1% BSA/0.1% Triton® X-100 detergent for 45 min and staining overnight at 4°C with appropriately diluted primary antibody. For immunoblotting, the best results may be obtained using whole cell or nuclear extracts; Sox2 may be difficult to detect in cytoplasmic extracts. The detection limit for recombinant Sox2 is ~5 ng/lane under reducing and non-reducing conditions. For intracellular staining by flow cytometry, fix cells in 4% paraformaldehyde and permeabilize with 0.1% saponin. Following fixation and permeabilization, dilute the antibody to 50 µg/ml and add 10 µl diluted antibody to 1-2.5 x 105 cells in a total volume of ≤200 µl. Following incubation with appropriate detection antibody, the cells should be washed a final time with 0.1% saponin prior to analysis. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of amino acids 135-317 of human Sox2, expressed in E. coli
ImmunogenHuman
Clone245610
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute with 200 µl sterile PBS for a final stock concentration of 500 µg/ml. Following reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage. Avoid freeze/thaw cycles of solutions. Thawed aliquots are stable for up to 1 month at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
SC1002-100UG 04055977209341

Documentation

Anti-Sox2 Mouse mAb (245610) MSDS

Title

Safety Data Sheet (SDS) 

Anti-Sox2 Mouse mAb (245610) Certificates of Analysis

TitleLot Number
SC1002

References

Hivatkozások áttekintése
Avilion, A.A., et al. 2003.Genes Dev. 17, 126.
Graham, V., et al. 2003.Neuron 39, 749.
Stevanovic, M. 2003.Mol. Biol. Rep. 30, 127.
Kishi, M., et al. 2000.Development 127, 791.
Uwanogho, D., et al. 1995.Mech. Dev. 49, 23.
Yuan, H., et al. 1995.Genes Dev. 9, 2635.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-January-2008 JSW
ApplicationImmunoblotting (1-2 µg/ml)
Immunocytochemistry (10 µg/ml; see comments)
Flow Cytometry (see comments)
DescriptionProtein G purified mouse monoclonal antibody derived by immunizing mice with the specified immunogen and fusing splenocytes with mouse myeloma cells. Recognizes the ~34 kDa Sox2 protein.
BackgroundSox2 is a member of the Sox (SRY-like HMG box) family of transcription factors, which exhibit diverse roles in development. Sox2 plays a role in specifying the first three lineages that are present at egg implantation. In addition, Sox2 functions in regulating proliferation and differentation in the developing peripheral nervous system. As a result of its role in development, Sox2, together with Oct4 and Nanog, is also essential for the propagation of undifferentiated stem cells.
HostMouse
Immunogen speciesHuman
Immunogena recombinant protein consisting of amino acids 135-317 of human Sox2, expressed in E. coli
Clone245610
IsotypeIgG2a
Specieshuman, mouse
Positive controlNTERA-2 cells
FormLyophilized
FormulationLyophilized from 0.2 µm-filter sterilized PBS, 5% trehalose.
CommentsFor immunocytochemistry, cells should be fixed in PBS containing 4% paraformaldehyde for 20 min, followed by blocking in PBS/10% normal donkey serum/1% BSA/0.1% Triton® X-100 detergent for 45 min and staining overnight at 4°C with appropriately diluted primary antibody. For immunoblotting, the best results may be obtained using whole cell or nuclear extracts; Sox2 may be difficult to detect in cytoplasmic extracts. The detection limit for recombinant Sox2 is ~5 ng/lane under reducing and non-reducing conditions. For intracellular staining by flow cytometry, fix cells in 4% paraformaldehyde and permeabilize with 0.1% saponin. Following fixation and permeabilization, dilute the antibody to 50 µg/ml and add 10 µl diluted antibody to 1-2.5 x 105 cells in a total volume of ≤200 µl. Following incubation with appropriate detection antibody, the cells should be washed a final time with 0.1% saponin prior to analysis. Antibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute with 200 µl sterile PBS for a final stock concentration of 500 µg/ml. Following reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage. Avoid freeze/thaw cycles of solutions. Thawed aliquots are stable for up to 1 month at 4°C.
Toxicity Standard Handling
ReferencesAvilion, A.A., et al. 2003.Genes Dev. 17, 126.
Graham, V., et al. 2003.Neuron 39, 749.
Stevanovic, M. 2003.Mol. Biol. Rep. 30, 127.
Kishi, M., et al. 2000.Development 127, 791.
Uwanogho, D., et al. 1995.Mech. Dev. 49, 23.
Yuan, H., et al. 1995.Genes Dev. 9, 2635.