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MABE1929-100UG Anti-Sox-11 Antibody, clone 19B2

Anti-Sox-11, clone 19B2, Cat. No. MABE1929, is a rat monoclonal antibody that detects Transcription Factor Sox-11 and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

MABE1929-100UG
100 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberMABE1929-100UG
DescriptionAnti-Sox-11 Antibody, clone 19B2
Alternate Names
  • Transcription factor SOX-11
Background InformationTranscription factor SOX-11 (UniProt: Q7M6Y2; also known as Sox-11) is encoded by the Sox11 (also known as Sox-11) gene (Gene ID: 20666) in murine species. Sox-11 is a neural transcription factor that is involved in the embryonic neurogenesis and plays a role in tissue modeling during development, neuronal cell proliferation, and survival. Its expression is prominent in the periventricular cells of the central nervous system. It is also observed in a wide range of tissues involved in epithelial-mesenchymal interactions. Sox-11 activity is controlled by epigenetics and transcriptional mechanisms and by post-translational modifications. Its three highly conserved phosphorylation sites, serines 30, 133, and 137, surrounding the DNA-binding high-mobility group (MMG box), are reported to modulate its transcriptional activity. These sites are reported to be unique to Sox-11. Serine 133 is shown to be specifically phosphorylated by protein kinase A. Higher expression of Sox-11 has been shown in Mantle cell lymphoma. Mice deficient in Sox-11 display defects in multiple organs including the lung, heart, and pancreas. (Ref.: Balta, EA., et al. (2018). Sci. Rep. 8; 16196; Mozos, A., et al. (2009). Haematologica. 94(11); 1555-1562).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Sox-11, clone 19B2, Cat. No. MABE1929, is a rat monoclonal antibody that detects Transcription Factor Sox-11 and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunofluorescence
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunofluorescence Analysis: 1:500 dilution from a representative lot detected Sox-11 in wild type C57BL/6 (E18.5) mouse brain tissues (Courtesy of Prof. Dr. Chichung Lie and Marie Theres Wittmann, Institut für Biochemie, FAU Erlangen-Nürnberg, Germany).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Sox-11 in Immunoprecipitation applications (Balta, E.A., et. al. (2018). Sci Rep. 8(1):16196).

Immunofluorescence Analysis: A representative lot detected Sox-11 in Immunofluorescence applications (Balta, E.A., et. al. (2018). Sci Rep. 8(1):16196).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to 113 amino acids from the C-terminal half of mouse Sox-11.
Clone19B2
Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 19B2 is a rat monoclonal antibody that detects murine SOX-11. It targets an epitope within the C-terminal half.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Sox11
  • Sox-11
Purification MethodProtein G purified
UniProt Number
Molecular Weight~38 kDa observed; 42.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in E11 whole mouse embryo tissue lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected Sox-11 in E11 whole mouse embryo tissue lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABE1929-100UG 04061842070022

Documentation

Anti-Sox-11 Antibody, clone 19B2 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Sox-11 Antibody, clone 19B2 Certificates of Analysis

TitleLot Number
Anti-Sox-11, clone 19B2 - Q3457394 Q3457394

References

Reference overviewPub Med ID
Phosphorylation of the neurogenic transcription factor SOX11 on serine 133 modulates neuronal morphogenesis.
Balta EA, Schäffner I, Wittmann MT, Sock E, von Zweydorf F, von Wittgenstein J, Steib K, Heim B, Kremmer E, Häberle BM, Ueffing M, Lie DC, Gloeckner CJ.
Sci Rep  8(1)  16196  2018

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