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AB5471P Anti-Semaphorin 3F Antibody

Anti-Semaphorin 3F Antibody is an antibody against Semaphorin 3F for use in IC & WB.

Anti-Semaphorin 3F Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Growth Cones & Axon Guidance Gene Symbol: SEMA3F, SEMAK, SEMA-IV, SEMA4, Sema4, sema4 UniProt Number: Q13275
AB5471P
100 µg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RICC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5471P
ReplacesAB5471
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Semaphorin 3F Antibody
References
Product Information
FormatAffinity Purified
HS Code3002 15 90
Control
  • Embryonic brain tissue, neural growth cones
PresentationAffinity Purified immunoglobulin. Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Semaphorin 3F Antibody is an antibody against Semaphorin 3F for use in IC & WB.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application NotesWestern blot: 0.5-1.0 μg/mL using ECL. Suggested antibody dilution buffer is TBST (10 mM Tris-HCl, pH 8.0, 150 mM NaCl with 0.05% Tween 20). Suggested incubation is for two hours at room temperature or overnight at 2-8°C.
Immunocytochemistry.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenSynthetic peptide from human Semaphorin 3F (Catalog number AG363). (TPWTQTQAVRFRFSRATDGALRPM).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes Semaphorin 3F (Sema3F), recombinant and purified protein. By Western blot the antibody recognizes a protein of 90 kDa in total extracts from tissue culture cells producing Sema3F. Semaphorin 3F is expressed ubiquitously with higher levels in lung, brain, spleen, muscle and heart.
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteThis antibody may also react with mouse because of extremely high sequence conservation in the SEM3F protein.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues (Kolodkin et al., 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 [PubMed 7637782]), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.[supplied by OMIM]
Gene Symbol
  • SEMA3F
  • SEMAK
  • SEMA-IV
  • SEMA4
  • Sema4
  • sema4
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q13275 # May play a role in cell motility and cell adhesion.
SIZE: 785 amino acids; 88381 Da
SUBCELLULAR LOCATION: Secreted (By similarity).
TISSUE SPECIFICITY: Expressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver.
DEVELOPMENTAL STAGE: Detected as early as embryonic day 10.
SIMILARITY: SwissProt: Q13275 ## Belongs to the semaphorin family. & Contains 1 Ig-like C2-type (immunoglobulin-like) domain. & Contains 1 Sema domain.
Molecular Weight90 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
AB5471P 04053252678332

Documentation

Anti-Semaphorin 3F Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Semaphorin 3F Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-SEMAPHORIN 3F AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2378767 2378767
RABBIT ANTI-SEMAPHORIN 3F AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2446932 2446932
RABBIT ANTI-SEMAPHORIN 3F - 3202254 3202254
RABBIT ANTI-SEMAPHORIN 3F - 3490886 3490886
RABBIT ANTI-SEMAPHORIN 3F -2757117 2757117
RABBIT ANTI-SEMAPHORIN 3F AFFINITY PURIFIED POLYCLONAL ANTIBODY 2909608
RABBIT ANTI-SEMAPHORIN 3F AFFINITY PURIFIED POLYCLONAL ANTIBODY 3076538

References

Reference overviewApplicationSpeciesPub Med ID
Expression of class 3 semaphorins and their receptors in human breast neoplasia.
Carolyn A Staton,Lucy A Shaw,Manoj Valluru,Leslie Hoh,Ivan Koay,Simon S Cross,Malcolm W Reed,Nicola J Brown
Histopathology  59  2010

Kivonat megmutatása 		21884206 21884206
Endogenous axon guiding chemorepulsant semaphorin-3F inhibits the growth and metastasis of colorectal carcinoma.
Wu, F; Zhou, Q; Yang, J; Duan, GJ; Ou, JJ; Zhang, R; Pan, F; Peng, QP; Tan, H; Ping, YF; Cui, YH; Qian, C; Yan, XC; Bian, XW
Clinical cancer research : an official journal of the American Association for Cancer Research  17  2702-11  2010

Kivonat megmutatása 		21349996 21349996
Soluble neuropilin-2, a nerve repellent receptor, is increased in rheumatoid arthritis synovium and aggravates sympathetic fiber repulsion and arthritis.
Alexander Fassold,Werner Falk,Sven Anders,Thomas Hirsch,Vladimir M Mirsky,Rainer H Straub
Arthritis and rheumatism  60  2009

Kivonat megmutatása 		19790074 19790074
Neuropilin-2 interacts with VEGFR-2 and VEGFR-3 and promotes human endothelial cell survival and migration
Favier, Benoit, et al.
Blood, 108:1243-1250 (2006)  2005

16621967 16621967
Identification of genes differentially expressed in rat alveolar type I cells
Dahlin, Katherine, et al.
Am. J. Respir. Cell Mol. Biol., 31:309-316 (2004)  2004

ImmunocytochemistryRat15205179 15205179