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ABN1662 Anti-STRA6

Anti-STRA6, Cat. No. ABN1662, is a highly specific rabbit polyclonal antibody that targets Stimulated by retinoic acid gene 6 protein (STRA6) and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-STRA6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Stra6 UniProt Number: O70491
ABN1662
100 µL  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFC, IH(P), IP, WBRbUnpurifiedPolyclonal Antibody
Description
Catalogue NumberABN1662
DescriptionAnti-STRA6
Alternate Names
  • Stimulated by retinoic acid gene 6 protein
  • Retinoic acid-responsive protein
Background InformationStimulated by retinoic acid gene 6 protein (UniProt: O70491; also known as Retinoic acid-responsive protein, STRA6) is encoded by the Stra6 gene (Gene ID: 20897) in murine species. STRA6 is a multi-pass membrane protein that acts as a high-affinity cell-surface receptor for the complex retinol-retinol binding protein (RBP/RBP4). It has one intramembrane and nine transmembrane helices in a dimeric assembly. It acts by removing retinol from RBP/RBP4 and transports it across the plasma membrane, where it can be metabolized. This mechanism does not depend on endocytosis. Binds to RBP/RBP4 with high affinity. Increases cellular retinol uptake from the retinol-RBP complex. STRA6 is widely expressed in the embryo and it in adults it is expressed choroid plexus and the brain microvascular cells that form the blood-organ barrier. It is also expressed in the retinal pigment epithelium, seminiferous epithelium in testis, the yolk sac, and in chorioallantoic placenta. Its expression in Sertoli cells depends on the stage of the spermatogenic cycle. STRA6 can be induced by retinoic acid and it is shown to be synergistically up-regulated by Wnt1 and retinoid in mammary epithelial cells. Mutations in Stra6 gene have been linked to the Matthew-Wood syndrome that is characterized by pulmonary agenesis/hypoplasia; microphthalmia/anophthalmia; and congenital cardiac, digestive, and urogenital malformations. (Ref.: Sadowski, S et al. (2017). Birth Defects Res. 109(4); 251-253; Chen, Y et al. (2016). Science 353 (6302), aad8266).
References
Product Information
FormatUnpurified
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-STRA6, Cat. No. ABN1662, is a highly specific rabbit polyclonal antibody that targets Stimulated by retinoic acid gene 6 protein (STRA6) and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunoprecipitation Analysis: A representative lot detected STRA6 in 293T cells transfected with an empty vector or pCMVTag2b-Stra6 (Courtesy of Dr. Noa Noy).

Western Blotting Analysis: A representative lot detected STRA6 in Western Blotting applications (Berry, D.C., et. al. (2012). Mol Cell Biol. 32(19):3851-9).

Western Blotting Analysis: A representative lot detected STRA6 in 293T cells transfected with an empty vector or pCMVTag2b-Stra6 (Courtesy of Dr. Noa Noy).

Flow Cytometry Analysis: A 1:100 dilution from a representative lot detected STRA6 in HT-29 cells.

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected STRA6 in mouse testis and human liver cancer tissues.
Biological Information
ImmunogenHis-tggged recombinant fragment corresponding to 109 amino acids from the C-terminal region of murine Stimulated by retinoic acid gene 6 (STRA6) protein.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects murine Stimulated by retinoic acid gene 6 protein (STRA6). it targets an epitope within 109 amino acids from the C-terminal region.
IsotypeIgG
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Stra6
Purification MethodUnpurified
UniProt Number
Molecular Weight~90 kDa observed; 73.77 kda calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH3T3/L1Adipocytes.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected STRA6 in NIH3T3/L1 Adipocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ABN1662 04054839396748