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ST1617 Anti-SPG3A Mouse mAb (1B9)

This Anti-SPG3A Mouse mAb (1B9) is validated for use in ELISA, Immunoblotting for the detection of SPG3A.

Anti-SPG3A Mouse mAb (1B9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Atlastin GTPase 1

ST1617
  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityHostAntibody Type
H, RMMonoclonal Antibody
Description
OverviewRecognizes the ~50 kDa SPG3A protein in PC-12 and IMR32 cells.
Catalogue NumberST1617
Brand Family Calbiochem®
SynonymsAnti-Atlastin GTPase 1
Application Data
Detection of rat SPG3A by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-SPG3A Mouse mAb (1B9) (Cat. No. ST1617) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human SPG3A by immunoblotting. Sample: Whole cell lysate from IMR32 cells. Primary antibody: Anti-SPG3A Mouse mAb (1B9) (Cat. No. ST1617) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.29.
Positive controlPC-12 cells, IMR32 cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 1-101 of human SPG3A, espressed as a GST fusion protein
Clone1B9
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ST1617 0

Documentation

Anti-SPG3A Mouse mAb (1B9) MSDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-November-2010 RFH
SynonymsAnti-Atlastin GTPase 1
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of rat SPG3A by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-SPG3A Mouse mAb (1B9) (Cat. No. ST1617) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human SPG3A by immunoblotting. Sample: Whole cell lysate from IMR32 cells. Primary antibody: Anti-SPG3A Mouse mAb (1B9) (Cat. No. ST1617) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~50 kDa SPG3A protein.
BackgroundThe protein encoded by this gene is a GTPase and a Golgi body transmembrane protein. The encoded protein can form a homotetramer and has been shown to interact with spastin and with mitogen-activated protein kinase kinase kinase kinase 4. This protein may be involved in axonal maintenance as evidenced by the fact that defects in this gene are a cause of spastic paraplegia type 3. Three transcript variants encoding two different isoforms have been found for this gene.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 1-101 of human SPG3A, espressed as a GST fusion protein
Clone1B9
IsotypeIgG₁
Specieshuman, rat
Positive controlPC-12 cells, IMR32 cells
FormLiquid
FormulationIn PBS, pH 7.29.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review