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MABF3081-25UG Anti-SLAMF6/NTB-A Antibody, clone NT-7

Anti-SLAMF6/NTB-A, clone NT-7 , Cat. No. MABF3081, is a mouse monoclonal antibody that detects NTB-A/CD352 and is tested for use in Flow Cytometry and ELISA.

ÚjMABF3081-25UG
25 μg  
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Áttekintés

Replacement Information
Description
Catalogue NumberMABF3081-25UG
DescriptionAnti-SLAMF6/NTB-A Antibody, clone NT-7
Alternate Names
  • SLAM family member 6
  • Activating NK receptor
  • NK-T-B-antigen (NTB-A)
  • CD352
  • Lymphocyte antigen 108
Background InformationSLAM family member 6 (UniProt: Q96DU3; also known as Activating NK receptor, NK-T-B-antigen (NTB-A), CD352) is encoded by the SLAMF6 (also known as KALI) gene (Gene ID: 114836) in human. SLAMF6 belongs to the 7-member signaling lymphocytic activation molecule family (SLAMF) of receptors broadly expressed in hematopoietic cells. SLAM receptors triggered by homo or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. SLAMF members carry one or more copies of immunoreceptor tyrosine-based switch motif or ITSM (T-X-Y-X-X-[VI]) in their cytoplasmic tails. Upon phosphorylation on the tyrosine residue, ITSM mediates the recruitment of SH2 domain-containing signaling molecules such as SLAM-associated protein (SAP), including SAP in T-, NK, and NK T-cells, EAT-2A and EAT-2B (murine) in NK cells and APCs. SLAMF6/NTB-A is predominantly localized to the cell membrane and is a single-pass type I membrane protein and is synthesized with a signal peptide sequence (aa 1-21), the removal of which yields the mature SLAMF6 with an extracellular domain (aa 22-226), a transmembrane domain (aa 227-247) and, a cytoplasmic domain (aa 248-331). Its expression is primarily observed in cells of the immune system. SLAMF6/NTB-A is a receptor on the surfaces of human NK, T, and B cells, mediating a signal whose malfunction may be involved in X-linked lymphoproliferative disease. Studies show that NTB-A is its own ligand and homophilic interaction of NTB-A enhances NK cell cytotoxicity and influences NK cell proliferation and IFN- secretion. It is also suggested that NTB-A is an interlymphocyte signaling molecule, which serves to orchestrate the activities of immune cells. Dysregulation of SLAMF6 signaling is associated with autoimmune conditions such as systemic lupus erythematosus (SLE), where it can contribute to abnormal immune cell activation. (Ref.: Flaig, R.M., et al. (2004). J Immunol. 172(11):6524-7; Stark, S., et al. (2005). J Immunol Methods. 296(1-2):149-58; Messmer, B., et al. (2006). J Immunol. 176(8):4646-50).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-SLAMF6/NTB-A, clone NT-7 , Cat. No. MABF3081, is a mouse monoclonal antibody that detects NTB-A/CD352 and is tested for use in Flow Cytometry and ELISA.
Key Applications
  • Flow Cytometry
  • ELISA
Application NotesTested Applications

ELISA: A representative lot detected NTB-A/CD352 in ELISA application (Stark, S., et al. (2005). J Immunol Methods. 296(1-2):149-58).

Flow Cytometry Analysis: A representative lot detected NTB-A/CD352 in Flow Cytometry application (Flaig, R.M., et al. (2004). J Immunol. 172(11):6524-7; Messmer, B., et al. (2006). J Immunol. 176(8):4646-50).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenRecombinant fragment from extracellular domain of human SLAMF6/NTB-A.
EpitopeExtracellular domain
CloneNT-7
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone NT-7 is a mouse monoclonal antibody that specifically detects SLAMF6/NTB-A. It targets an epitope within the extracellular domain.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SLAMF6
Purification MethodProtein G purified
UniProt Number
Molecular WeightTarget molecular weight; 37.34 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis: 1.0ug of this antibody detected SLAMF6/NTB-A in one million Human peripheral blood mononuclear cells (PBMC).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABF3081-25UG 04065272290227

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