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MABE145 Anti-Runx3 Antibody, clone R3-5G4

Use Anti-Runx3 Antibody, clone R3-5G4 (mouse monoclonal antibody) validated in WB, IP to detect Runx3 also known as Runt-related transcription factor 3, Acute myeloid leukemia 2 protein, PEA2-alpha C, PEBP2-alpha C.

Anti-Runx3 Antibody, clone R3-5G4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: RUNX3, AML2, CBFA3, PEBP2A3 UniProt Number: Q13761
MABE145
100 µL  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE145
DescriptionAnti-Runx3 Antibody, clone R3-5G4
Alternate Names
  • Runt-related transcription factor 3
  • Acute myeloid leukemia 2 protein
  • Core-binding factor subunit alpha-3
  • CBF-alpha-3
  • Oncogene AML-2
  • Polyomavirus enhancer-binding protein 2 alpha C subunit
  • PEA2-alpha C
  • PEBP2-alpha C
  • SL3-3 enhancer factor 1 alpha C subunit
  • SL3/AKV core-binding factor alpha C subunit
Background InformationRunx3 is a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer.
References
Product Information
FormatPurified
Control
  • Daudi cell lysate.
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-Runx3 Antibody, clone R3-5G4 (mouse monoclonal antibody) validated in WB, IP to detect Runx3 also known as Runt-related transcription factor 3, Acute myeloid leukemia 2 protein, PEA2-alpha C, PEBP2-alpha C.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: A representative lot from an independent laboratory detected Runx3 in HCT116, SW620, and SW480 nuclear extracts. (Ito, K., et al. (2008). Cancer Cell. 14(3):226-237.)
Biological Information
ImmunogenRecombinant protein corresponding to human Runx3.
CloneR3-5G4
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RUNX3
  • AML2
  • CBFA3
  • PEBP2A3
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.

SUBUNIT STRUCTURE Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK.

SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: The tyrosine phosphorylated form localizes to the cytoplasm.
Molecular Weight~44 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in Daudi cell lysate.

Western Blot Analysis: 0.25 mg/mL of this antibody detected Runx3 in 10 µg Daudi cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABE145 04053252427169

Documentation

Anti-Runx3 Antibody, clone R3-5G4 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Runx3 Antibody, clone R3-5G4 Certificates of Analysis

TitleLot Number
Anti-Runx3, clone R3-5G4 - 2360879 2360879
Anti-Runx3, clone R3-5G4 - 2289165 2289165
Anti-Runx3, clone R3-5G4 - 3240479 3240479
Anti-Runx3, clone R3-5G4 - NRG1953328 NRG1953328
Anti-Runx3, clone R3-5G4 -2893216 2893216
Anti-Runx3, clone R3-5G4 Monoclonal Antibody 3084595
Anti-Runx3, clone R3-5G4 Monoclonal Antibody 2911996
Anti-Runx3, clone R3-5G4 Monoclonal Antibody 2935455

References

Reference overviewPub Med ID
RUNX3 attenuates beta-catenin/T cell factors in intestinal tumorigenesis.
Ito, Kosei, et al.
Cancer Cell, 14: 226-37 (2008)  2008

Kivonat megmutatása 		18772112 18772112

Brochure

Title
New Products: Volume 3, 2012