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05-1362 Anti-RING1 Antibody, clone 4D6

Use Anti-RING1 Antibody, clone 4D6 (Mouse Monoclonal Antibody) validated in WB, ICC to detect RING1 also known as Polycomb complex protein RING1.

Anti-RING1 Antibody, clone 4D6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: EC 6.3.2.- , OTTHUMP00000029416, RING1A , RNF1 UniProt Number: Q06587
05-1362
100 µg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1362
DescriptionAnti-RING1 Antibody, clone 4D6
Alternate Names
  • Polycomb complex protein RING1
  • ring finger protein 1
Background InformationRING1 belongs to the RING finger family, members of which are characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain.
References
Product Information
FormatPurified
Control
  • HEK293 cell lysate
PresentationPurified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-RING1 Antibody, clone 4D6 (Mouse Monoclonal Antibody) validated in WB, ICC to detect RING1 also known as Polycomb complex protein RING1.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry: Representative lot data.
A 1:500 dilution of a previous lot was used in immunocytochemistry on NIH/3T3 and A431 cells.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to RING1.
Clone4D6
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody detects RING1.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq].
Gene Symbol
  • EC 6.3.2.-
  • OTTHUMP00000029416
  • RING1A
  • RNF1
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.

PATHWAY: Protein modification; protein ubiquitination.

SUBUNIT STRUCTURE: Component of some chromatin-associated Polycomb complex (PcG). Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2/RING2 MBLR, L3MBTL2 and YAF2. Interacts with CBX2 and PCGF6. Component of chromatin-associated class II PcG repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of RYBP, PCGF1, BCOR and RNF2/RING2.

SUBCELLULAR LOCATION: Nucleus speckle.

SEQUENCE SIMILARITIES: Contains 1 RING-type zinc finger.
Molecular Weight~42 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by western blot on HEK293 cell lysate.

Western Blot Analysis: 0.5-2 µg/mL of this antibody detected RING1 in HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
05-1362 04053252369513

Documentation

Anti-RING1 Antibody, clone 4D6 MSDS

Title

Safety Data Sheet (SDS) 

Anti-RING1 Antibody, clone 4D6 Certificates of Analysis

TitleLot Number
Anti-RING1, clone 4D6 - 2426530 2426530
Anti-RING1, clone 4D6 - 3128853 3128853
Anti-RING1, clone 4D6 - NG1620991 NG1620991
Anti-RING1, clone 4D6 - NG1698996 NG1698996
Anti-RING1, clone 4D6 - NG1829859 NG1829859
Anti-RING1, clone 4D6 - NG1882240 NG1882240
Anti-RING1, clone 4D6 -2531915 2531915
Anti-RING1, clone 4D6 -2697406 2697406

References

Reference overviewPub Med ID
Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications.
van de Werken, C; van der Heijden, GW; Eleveld, C; Teeuwssen, M; Albert, M; Baarends, WM; Laven, JS; Peters, AH; Baart, EB
Nature communications  5  5868  2014

Kivonat megmutatása 		25519718 25519718