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07-1814 Anti-RCC1 (Trimethyl Ser2) Antibody

Anti-RCC1 (Trimethyl Ser2) Antibody is a Rabbit Polyclonal Antibody for detection of RCC1 also known as regulator of chromosome condensation 1 & has been validated in WB, ICC.

Anti-RCC1 (Trimethyl Ser2) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: RCC1, SHEP1, CHC1, RCC1-I, HERC2 UniProt Number: P18754
07-1814
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1814
DescriptionAnti-RCC1 (Trimethyl Ser2) Antibody
Alternate Names
  • regulator of chromosome condensation 1
  • chromosome condensation 1
  • Cell cycle regulatory protein
  • Chromosome condensation protein 1
  • guanine nucleotide-releasing protein
Background InformationThe Regulator of Chromosome Condensation 1 (RCC1) is a critical cell cycle regulator. This protein is involved in regulating the onset of chromosome condensation during S phase. The RCC1 protein binds to chromatin and interacts with Ran, a nuclear GTP-binding protein. This complex acts as a guanine-nucleotide dissociation stimulator (GDS) by promoting the loss of bound GDP and the uptake of fresh GTP.
References
Product Information
FormatAffinity Purified
Control
  • HEPG2 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-RCC1 (Trimethyl Ser2) Antibody is a Rabbit Polyclonal Antibody for detection of RCC1 also known as regulator of chromosome condensation 1 & has been validated in WB, ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application Notes Immunocytochemistry
A representitive lot of this antibody was reported to work in Ic. See Chen, T., et al. (2007) Nat Cell Biol, 9(5):596-603.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human RCC1.
EpitopeUnknown
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes RCC1 methylated at serine 2. Moderate cross reactivity with the unmodified form of the protein has been observed.
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteDemonstrated to react with human. Predicted to react with mouse and rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • RCC1
  • SHEP1
  • CHC1
  • RCC1-I
  • HERC2
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds to the chromatin. RCC1/Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA.

SUBUNIT STRUCTURE: Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.

SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Becomes dispersed throughout the cytoplasm during mitosis.

MISCELLANEOUS: Patients with Raynaud disease produce antibodies that bind to RCC1.

SEQUENCE SIMILARITIES: Contains 7 RCC1 repeats.
Molecular Weight~ 50 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HEPG2 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected RCC1 on 10 µg of HEPG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
07-1814 04053252517471