Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

AB9714-I Anti-RAGE

Anti-RAGE, Cat. No. AB9714-I, is a highly specific rabbit polyclonal antibody that targets Advanced glycosylation end product-specific receptor and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-RAGE MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: AGER, RAGE UniProt Number: Q15109
AB9714-I
100 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, MWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB9714-I
ReplacesAB9714
DescriptionAnti-RAGE
Alternate Names
  • Advanced glycosylation end product-specific receptor
  • Receptor for advanced glycosylation end products
  • RAGE
Background InformationAdvanced glycosylation end product-specific receptor (UniProt: Q15109; also known as Receptor for advanced glycosylation end products, RAGE) is encoded by the AGER (also known as RAGE) gene (Gene ID: 177) in human. RAGE is a single-pass type I membrane protein that contains two Ig-like C2-type domains, one Ig-like V-type domain, a 21 amino acid transmembrane domain and a 41 amino acids cytoplasmic domain. RAGE mediates interactions of advanced glycosylation end products (AGE), which are non-enzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. RAGE acts as a mediator of both acute and chronic vascular inflammation in conditions, such as atherosclerosis and in complication of diabetes. Blockage of RAGE is reported to suppress development of atherosclerosis and vascular disorders. RAGE is expressed by endothelium, mononuclear phagocytes, neurons and smooth muscle cells. Higher expression of RAGE is observed during development, especially in the central nervous system, and its levels decline during maturity. Besides binding to advanced glycosylation end product (AGE), RAGE also binds amyloid beta peptide, S100family proteins, and leukocyte integrins. Excessive amounts of amyloid peptides (Ab) in brain leads to the hyperstimulation of RAGE. The RAGE-Ab interaction is thought to result in oxidative stress leading to neuronal degeneration.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-RAGE, Cat. No. AB9714-I, is a highly specific rabbit polyclonal antibody that targets Advanced glycosylation end product-specific receptor and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected RAGE in human lung tissue.
Biological Information
ImmunogenKLH-cojugated linear peptide corresponding to 20 amino acids from the N-terminal region of human RAGE.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects Receptor for Advanced Glycosylation End Products (RAGE). This antibody targets an epitope corresponding to 21 amino acids in the N-terminal region of human RAGE. Human, mouse, and rat RAGE display 95% homology in this epitope region.
Species Reactivity
  • Rat
  • Mouse
Species Reactivity NoteRat, Mouse. Predicted to react with Human based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • AGER
  • RAGE
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~48 kDa observed; 42.80 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse lung tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected RAGE in 10 µg of mouse lung tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
AB9714-I 04054839167522