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MABF1967B-100UG Anti-RABV Glycoprotein Antibody, biotinylated Antibody, serum free Antibody, clone D1-25

MABF1967B-100UG
100 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberMABF1967B-100UG
DescriptionAnti-RABV Glycoprotein Antibody, biotinylated Antibody, serum free Antibody, clone D1-25
Alternate Names
  • Glycoprotein
Background InformationGlycoprotein (UniProt: P08667; also known as RABV glycoprotein) is encoded by the G gene (Gene ID: 1489856) in rabies virus (strain Pasteur vaccins). Rabies virus glycoprotein is a single-pass type I membrane protein that attaches the virus to host cellular receptors, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. It has been reported that the muscular form of nicotinic ACh receptor, p75 neurotrophin receptor (p75NTR) and the neuronal cell adhesion molecule can bind to glycoprotein and facilitate the entry of rabies virus into cells. Glycoprotein is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. Its structure includes a virion surface (aa 20-459), helical region (aa 460-480) and an intravirion (aa 481-524) domain. Arginine 352 is reported to be involved in Rabies virus pathogenicity and its mutation attenuates the virus. Glycoprotein is glycosylated and palmitoylated by host and glycosylation is considered to be crucial for glycoprotein export at the cell surface. Clone D1-25 recognizes an epitope in antigenic site III on the rabies virus (RABV) glycoprotein. (Ref.: Nagarajan, T., et al. (2006). Biologicals. 34(1); 21-27; Müller, T., et al. (2009). PLoS Negl. Trop. Dis. 3(11); e542; Sissoëff,1, L., et al. (2005). J. Gen. Virol. 86(9); 2543-2552).
References
Product Information
FormatBiotin
HS Code3002 15 90
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Applications
ApplicationAnti-RABV Glycoprotein, biotinylated, serum free, clone D1-25 , Cat. No. MABF1967B, is a mouse monoclonal antibody that detects Rabies virus Glycoprotein and has been tested for use in ELISA, Immunoprecipitation. and Neutralization.
Key Applications
  • ELISA
  • Immunoprecipitation
  • Neutralization Assay
Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated RABV Glycoprotein in Immunoprecipitation applications (Sissoeff, L., et. al. (2005). J Gen Virol. 86(Pt 9):2543-52; Desmezieres, E., et. al. (2003). Virus Res. 91(2):181-7).

ELISA Analysis (ELISA): ELISA Analysis: A representative lot detected RABV Glycoprotein in ELISA applications (Nagarajan, T., et. al. (2006). Biologicals. 34(1):21-7; Morgeaux, S., et. al. (2017). Vaccine. 35(6):966-971; Korimbocus, J., et. al. (2016). Vaccine. 34(28):3310-6; Gilbert, R., et. al. (2013). Vaccine. 31(50):6022-9; Fournier-Caruana, J., et. al. (2003). Biologicals. 31(1):9-16).

Neutralizing Analysis: A representative lot neutralized Rabies virus in Neutralizing applications. (Muller, T., et. al. (2009). PLoS Negl Trop Dis. 3(11):e542; Sissoeff, L., et. al. (2005). J Gen Virol. 86(Pt 9):2543-52; Morgeaux, S., et. al. (2017). Vaccine. 35(6):966-971; Fournier-Caruana, J., et. al. (2003). Biologicals. 31(1):9-16).
Biological Information
ImmunogenInactivated and purified Rabies Virus (Pasteur virus strain).
CloneD1-25
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone D1-25 is a mouse monoclonal antibody that detects Rabies virus glycoprotein. It targets an epitope within nine amino acids in the antigenic site III.
IsotypeIgG1κ
Species Reactivity
  • Virus
Species Reactivity NoteRabies Virus.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • G
Purification MethodProtein G purified
UniProt Number
Molecular Weight58.56 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABF1967B-100UG 04061841134947

Documentation

Anti-RABV Glycoprotein Antibody, biotinylated Antibody, serum free Antibody, clone D1-25 MSDS

Title

Safety Data Sheet (SDS) 

Anti-RABV Glycoprotein Antibody, biotinylated Antibody, serum free Antibody, clone D1-25 Certificates of Analysis

TitleLot Number
Anti-RABV Glycoprotein, biotinylated, serum free, clone D1-25 - 3401504-1 3401504-1
Anti-RABV Glycoprotein, biotinylated, serum free, clone D1-25 - 3654157 3654157
Anti-RABV Glycoprotein, biotinylated, serum free, clone D1-25 - 3708042 3708042
Anti-RABV Glycoprotein, biotinylated, serum free, clone D1-25 - 3988491 3988491
Anti-RABV Glycoprotein, biotinylated, serum free, clone D1-25 - 4023312 4023312
Anti-RABV Glycoprotein, biotinylated, serum free, clone D1-25 - 4045889 4045889
Anti-RABV Glycoprotein, biotinylated, serum free, clone D1-25 - 4116941 4116941
Anti-RABV Glycoprotein, biotinylated, serum free, clone D1-25 - 4124277 4124277
Anti-RABV Glycoprotein, biotinylated, serum free, clone D1-25 - 4128023 4128023
Anti-RABV Glycoprotein, biotinylated, serum free, clone D1-25 - 4140002 4140002

References

Reference overviewPub Med ID
Replacement of in vivo human rabies vaccine potency testing by in vitro glycoprotein quantification using ELISA - Results of an international collaborative study.
Morgeaux, S; Poirier, B; Ragan, CI; Wilkinson, D; Arabin, U; Guinet-Morlot, F; Levis, R; Meyer, H; Riou, P; Shaid, S; Volokhov, D; Tordo, N; Chapsal, JM
Vaccine  35  966-971  2016

Kivonat megmutatása
28081969 28081969
Development and validation of a quantitative competitive ELISA for potency testing of equine anti rabies sera with other potential use.
Korimbocus, J; Dehay, N; Tordo, N; Cano, F; Morgeaux, S
Vaccine  34  3310-6  2015

Kivonat megmutatása
27151880 27151880
A relevant in vitro ELISA test in alternative to the in vivo NIH test for human rabies vaccine batch release.
Gibert, R; Alberti, M; Poirier, B; Jallet, C; Tordo, N; Morgeaux, S
Vaccine  31  6022-9  2013

Kivonat megmutatása
24161572 24161572
Development of a mouse monoclonal antibody cocktail for post-exposure rabies prophylaxis in humans.
Müller, T; Dietzschold, B; Ertl, H; Fooks, AR; Freuling, C; Fehlner-Gardiner, C; Kliemt, J; Meslin, FX; Franka, R; Rupprecht, CE; Tordo, N; Wanderler, AI; Kieny, MP
PLoS Negl Trop Dis  3  e542  2009

Kivonat megmutatása
19888334 19888334
A simple immuno-capture ELISA to estimate rabies viral glycoprotein antigen in vaccine manufacture.
Nagarajan, T; Reddy, GS; Mohana Subramanian, B; Rajalakshmi, S; Thiagarajan, D; Tordo, N; Jallet, C; Srinivasan, VA
Biologicals  34  21-7  2005

Kivonat megmutatása
16188454 16188454
Stable trimerization of recombinant rabies virus glycoprotein ectodomain is required for interaction with the p75NTR receptor.
Sissoëff, L; Mousli, M; England, P; Tuffereau, C
J Gen Virol  86  2543-2552  2004

Kivonat megmutatása
16099913 16099913
Differential stability and fusion activity of Lyssavirus glycoprotein trimers.
Desmézières, E; Maillard, AP; Gaudin, Y; Tordo, N; Perrin, P
Virus Res  91  181-7  2003

Kivonat megmutatása
12573496 12573496
Inactivated rabies vaccine control and release: use of an ELISA method.
Fournier-Caruana, J; Poirier, B; Haond, G; Jallet, C; Fuchs, F; Tordo, N; Perrin, P
Biologicals  31  9-16  2003

Kivonat megmutatása
12623055 12623055