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ABN38 Anti-RAB35 Antibody

This Anti-RAB35 Antibody is validated for use in WB, IH(P) for the detection of RAB35.

Anti-RAB35 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: RAB35, RAB1C, RAY UniProt Number: Q15286
ABN38
100 µg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, Ca, EqWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN38
DescriptionAnti-RAB35 Antibody
Alternate Names
  • Ras-related protein Rab-35
  • GTP-binding protein RAY
  • Ras-related protein Rab-1C
Background InformationRAB35 is a member of the Rab GTPases identified in the human genome. It is ubiquitously expressed and regulates a fast endocytotic recycling events associated with the terminal steps of cytokinesis, the formation of immunological synapse, and peptide loading onto major histocompatibility complex III molecules. RAB35 also plays a significant role in the formation of the immunological synapse (IS) between peptide-loaded antigen presenting cells (APCs) and T cells.
References
Product Information
FormatAffinity Purified
Control
  • Human brain tissue lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-RAB35 Antibody is validated for use in WB, IH(P) for the detection of RAB35.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:500 dilution from a representative lot detected RAB35 in normal human brain tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human RAB35.
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes RAB35 at the C-terminus.
Species Reactivity
  • Human
  • Rat
  • Canine
  • Horse
Species Reactivity NoteDemonstrated to react with Human and Rat. Predicted to react with Canine and Equine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • RAB35
  • RAB1C
  • RAY
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge.

SUBUNIT STRUCTURE: Interacts with DENND1A and DENND1B in a nucleotide-dependent manner. The low binding to DENND1C is nucleotide-independent. All 3 DENND1 act as GEFs for RAB35 and thus activate the protein.

SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Membrane › clathrin-coated pit. Cytoplasmic vesicle › clathrin-coated vesicle. Endosome. Melanosome. Note: Present on sorting endosomes and endosomal tubules. Tends to be enriched in PIP2-positive cell membrane domains. During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

SEQUENCE SIMILARITIES: Belongs to the small GTPase superfamily. Rab family.
Molecular Weight~19 kDa observed. The calculated molecular weight is 23 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in human brain tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected RAB35 on 10 µg of human brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ABN38 04053252423703