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05-488-I-100UG Anti-Pyk2/CAKβ Antibody, clone 74

Anti-Pyk2/CAKβ, clone 74, Cat. No. 05-488-I, is a mouse monoclonal antibody that detects Protein-tyrosine kinase 2b and is tested for use in Immunocytochemistry and Western Blotting.

05-488-I-100UG
100 μg  
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Replacement Information
Description
Catalogue Number05-488-I-100UG
DescriptionAnti-Pyk2/CAKβ Antibody, clone 74
Alternate Names
  • Protein-tyrosine kinase 2-beta
  • EC:2.7.10.2
  • Calcium-dependent tyrosine kinase
  • CADTK
  • Calcium-regulated non-receptor proline-rich tyrosine kinase
  • Cell adhesion kinase beta
  • CAK-beta
  • CAKB
  • Focal adhesion kinase 2
  • FADK 2
  • Proline-rich tyrosine kinase 2
Background InformationProtein-tyrosine kinase 2β (UniProt: P70600; also known as EC:2.7.10.2, Calcium-dependent tyrosine kinase, CADTK, Calcium-regulated non-receptor proline-rich tyrosine kinase, Cell adhesion kinase beta, CAK-beta, CAKB, Focal adhesion kinase 2, FADK 2, Proline-rich tyrosine kinase 2, Pyk2) is encoded by the Ptk2b (also known as Pak2, Pyk2) gene (Gene ID: 50646) in rat. Pky2 is a non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading, and bone remodeling. Its protein kinase domain is localized in amino acids 425-683. Pyk2 plays a role in the regulation of the humoral immune response and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. It is highly expressed in pulmonary vein endothelial cells, lung, and brain. It localizes to the cytoplasmic side of the membrane. Pyk2 is activated by phosphorylation of tyrosine residues in response to various stimuli that elevate intracellular calcium levels. Tyr402 is the major autophosphorylation site, but it can also be phosphorylated by other kinases. This phosphorylation promotes interaction with Src and Src family members, leading to phosphorylations at Tyr579, Tyr580, and Tyr881. Phosphorylation at Tyr402, Tyr579 and Tyr580 is required for its optimal kinase activity and phosphorylation at Tyr881 is essential for its interaction with Grb2.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Pyk2/CAKβ, clone 74, Cat. No. 05-488-I, is a mouse monoclonal antibody that detects Protein-tyrosine kinase 2b and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A 1:250 dilution from a representative lot detected Pyk2/CAKβ in rat brain tissue lysate.

Western Blotting Analysis: A representative lot detected Pyk2/CAKβ in Western Blotting applications (Bernard-Trifilo, J.A., et. al. (2005). J Neurochem. 93(4):834-49).

Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected Pyk2/CAKβ in E18 rat cortex neurons.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 412 amino acids from the N-terminal half of rat Protein-tyrosine kinase 2-beta (Pyk2).
EpitopeN-terminal half
Clone74
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 74 is a mouse monoclonal antibody that detects Protein-tyrosine kinase 2β (Pyk2). It targets an epitope within the N-terminal half.
IsotypeIgG1
Species Reactivity
  • Rat
  • Human
Species Reactivity NoteHuman, Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Ptk2b
  • Pak2
  • Pyk2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~110 kDa observed; 115.78 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected Pyk2/CAKβ in Raji cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
05-488-I-100UG 04065265944854