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AB10180 Anti-Pumilio-1 (PUM1) Antibody

This Anti-Pumilio-1 (PUM1) Antibody is validated for use in WB, IC for the detection of Pumilio-1 (PUM1).

Anti-Pumilio-1 (PUM1) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Hematopoietic Stem Cells, RNA Metabolism & Binding Proteins Gene Symbol: PUM1, HSPUM, HsPUM, PUMH, PUMH1, PUML1, Pumilio-1 UniProt Number: Q14671
AB10180
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, B, Rhesus Macaque, Chp, PmWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB10180
DescriptionAnti-Pumilio-1 (PUM1) Antibody
Alternate Names
  • pumilio (Drosophila) homolog 1
  • pumilio 1
  • pumilio homolog 1 (Drosophila)
Background InformationWithin the mammalian genome are two genes belonging to a family of housekeeping genes known as PUF. These genes are known as PUM1 and PUM2. PUF proteins are characterized by having a C-terminal RNA-binding domain called the Pumilio homolog domain, which affects the stability of mRNA and translation by binding the 3’-UTR of mRNA targets. PUM plays an active role in the sustainability of mitotic stem cell proliferation and the regulation of cell development and differentiation. Very little has been discovered about the specific role for PUM1. However it has been shown to bind an mRNA sequence known as Nanos response element and to interact with RNA. PUM1 is found in heart, brain, muscle, kidney, stomach and intestine. It is not expressed in putamen, hippocampus, medulla oblangata, corpus collasum, cerebellum and caudate nucleus.
References
Product Information
FormatAffinity Purified
Control
  • H9 human stem cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
ApplicationThis Anti-Pumilio-1 (PUM1) Antibody is validated for use in WB, IC for the detection of Pumilio-1 (PUM1).
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Pumilio-1 in human and mouse embryonic stem cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Pumilio-1.
EpitopeN-terminus
HostRabbit
SpecificityThis antibody recognizes the N-terminus of Pumilio-1 (PUM1).
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Bovine
  • Rhesus Macaque
  • Chimpanzee
  • Primate
Species Reactivity NoteDemonstrated to react with human, mouse, and rat.
Predicted to react with bovine, rhesus monkey, chimpanzee, and primate based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq].
Gene Symbol
  • PUM1
  • HSPUM
  • HsPUM
  • PUMH
  • PUMH1
  • PUML1
  • Pumilio-1
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. May be required to support proliferation and self-renewal of stem cells By similarity.

SUBUNIT STRUCTURE: Binds in a sequence-specific manner to the 3'-UTR of some mRNAs.

SUBCELLULAR LOCATION: Cytoplasm Probable.

TISSUE SPECIFICITY: Expressed in brain, heart, kidney, muscle, intestine and stomach. Not expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.

INDUCTION: Strongly down-regulated in keratinocytes upon ultraviolet B radiation.

DOMAIN: The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half donut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs.

SEQUENCE SIMILARITIES: Contains 1 PUM-HD domain.

Contains 8 pumilio repeats.
Molecular Weight~120 kDa.
UniProtKB/Swiss-Prot Q14671 (PUM1_HUMAN) entry describes 2 isoforms produced by alternative splicing: Isoform 1 at ~126 kDa and Isoform 2 at ~124 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in H9 human stem cell lysate.

Western Blot Analysis: A 1:5,000 dilution of this antibody detected Pumilio-1 (PUM1) in 10 µg of H9 human stem cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
AB10180 04053252699863