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PC548 Anti-Porin (Ab-5) (185-197) Rabbit pAb

This Anti-Porin (Ab-5) (185-197) Rabbit pAb is validated for use in Immunoblotting for the detection of Porin (Ab-5) (185-197).

Anti-Porin (Ab-5) (185-197) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-VDAC (Voltage-Dependent Anion Channel)

PC548
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityHostAntibody Type
H, RRbPolyclonal Antibody

Products

KatalógusszámCsomagolás Menny./csomag
PC548-50UG Muanyagampulla 50 μg
Description
OverviewRecognizes the ~31 kDa porin protein.
Catalogue NumberPC548
Brand Family Calbiochem®
SynonymsAnti-VDAC (Voltage-Dependent Anion Channel)
Application Data
Detection of rat porin by immunoblotting. Sample: Whole tissue extract from heart. Primary antibody: Anti-Porin (Ab-5) (185-197) Rabbit pAb (Cat. No. PC548) (2 µg/ml). Detection: chemiluminescence
References
ReferencesCrompton, M., et al. 1999. Biochem. J. 341, 233.
Yu, W.H., et al. 1995. J. Biol. Chem. 270, 13998.
McEnry, M.W., et al. 1993. J. Biol. Chem. 268, 23289.
Product Information
FormLiquid
FormulationIn PBS, 1 mg/ml BSA.
Positive controlRat heart tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (2 µg/ml)
Application CommentsThe immunogen peptide is 100% conserved in all three isoforms of VDAC (VDAC1, VDAC2, and VDAC3). Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 185-197 of human porin
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
PC548-50UG 04055977207781

Documentation

Anti-Porin (Ab-5) (185-197) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-Porin (Ab-5) (185-197) Rabbit pAb Certificates of Analysis

TitleLot Number
PC548

References

Hivatkozások áttekintése
Crompton, M., et al. 1999. Biochem. J. 341, 233.
Yu, W.H., et al. 1995. J. Biol. Chem. 270, 13998.
McEnry, M.W., et al. 1993. J. Biol. Chem. 268, 23289.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-October-2007 RFH
SynonymsAnti-VDAC (Voltage-Dependent Anion Channel)
ApplicationImmunoblotting (2 µg/ml)
Application Data
Detection of rat porin by immunoblotting. Sample: Whole tissue extract from heart. Primary antibody: Anti-Porin (Ab-5) (185-197) Rabbit pAb (Cat. No. PC548) (2 µg/ml). Detection: chemiluminescence
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~31 kDa (VDAC) porin protein.
BackgroundThe Voltage-Dependent Anion Channel (VDAC or mitochondrial Porin) is a constituent of the outer mitochondrial membrane. The VDAC protein is thought to form the major pores through which adenine nucleotides are transferred through the outer mitochondrial membrane. VDAC has also been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. There are also strong indications that the VDAC-adenine nucleotide translocase-CyP-D complex can recruit a number of other proteins, including Bax, and that the complex is utilized in some capacity during apoptosis.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids 185-197 of human porin
IsotypeIgG
Specieshuman, rat
Positive controlRat heart tissue
FormLiquid
FormulationIn PBS, 1 mg/ml BSA.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThe immunogen peptide is 100% conserved in all three isoforms of VDAC (VDAC1, VDAC2, and VDAC3). Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesCrompton, M., et al. 1999. Biochem. J. 341, 233.
Yu, W.H., et al. 1995. J. Biol. Chem. 270, 13998.
McEnry, M.W., et al. 1993. J. Biol. Chem. 268, 23289.