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MABS1255 Anti-Platelet Factor 4 Antibody, clone 197.2

Detect PF4 using this mouse monoclonal Anti-Platelet Factor 4, clone 197.2, Cat. No. MABS1255, validated for use in ELISA, Flow Cytometry, Function Analysis, Immunocytochemistry. and Western Blotting.

Anti-Platelet Factor 4 Antibody, clone 197.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: PF4, CXCL4, SCYB4 UniProt Number: P02776
MABS1255
100 μg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, FC, ICC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1255
DescriptionAnti-Platelet Factor 4 Antibody, clone 197.2
Alternate Names
  • Platelet factor 4
  • C-X-C motif chemokine 4
  • Iroplact
  • Oncostatin-A
  • PF-4
Background InformationPlatelet factor 4 (UniProt P02776; also known as C-X-C motif chemokine 4, Iroplact, Oncostatin-A, PF-4) is encoded by the PF4 (also known as CXCL4, SCYB4) gene (Gene ID 5196) in human. Platelet factor 4 (PF4) is a small CXC chemokine family cytokine released from alpha-granules of activated platelets during platelet aggregation to promote blood coagulation. PF4 binds heparin with high affinity (Kd ~4-20 nM) via its heparin-binding domain (a.a. 92-98) and neutralizes the anti-thrombin activity of heparin-like molecules. PF4 is chemotactic for neutrophils, fibroblasts and monocytes, and interacts with the chemokine receptor CXCR3 splice variant CXCR3B. In individuals suffering from heparin-induced thrombocytopenia (HIT), PF4-heparin complexes can trigger an immune response and the generation of anti-PF4 antibodies, resulting in the foramtion of macromolecular antibody-heparin-PF4 immune complexes that bind Fc RIIa (CD32a) on platelets and induce platelet activation. Platelet activation in turn results in more PF4 release into circulation, enhanced immune-complex formation, exaggerated platelet activation, and cell clearance. PF4 is synthesized with a signal peptide sequence (a.a. 1-31) that is removed posttranslationally to produce the mature PF4 (a.a. 32-101), additional proteolytic cleavage yields the PF4 short form (a.a. 48-101).
References
Product Information
FormatPurified
PresentationPurified mouse IgG1 in buffer containing PBS without preservatives.
Quality LevelMQ100
Applications
ApplicationDetect PF4 using this mouse monoclonal Anti-Platelet Factor 4, clone 197.2, Cat. No. MABS1255, validated for use in ELISA, Flow Cytometry, Function Analysis, Immunocytochemistry. and Western Blotting.
Key Applications
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected E. coli expressed recombinant mature human PF4 (a.a. 32-101) under both reduced and non-reduced condition (Courtesy of Dr. Richard H. Aster, MD, PhD, Blood Center of Wisconsin, U. S. A.).

ELISA Analysis: A representative lot detected PF4 in the absence of heparin by ELISA. Decreased immunoreactivity of clone 197.2 was seen toward heparin-complexed PF4 (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).

Function Analysis: A representative lot and PF4, but not clone 197.2 or PF4 alone, activated human neutrophils as indicated by Mac-1 upregulation (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).

Immunocytochemistry Analysis: A representative lot, preconjugated with Alexa Fluor® 488, immunolabeled the co-administered PF4 on human neutrophils. CD32a (Fc gamma RIIA) was seen co-localized with the PF4 anti-PF4 immune complexes on the surface of activated neutrophils (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).

Western Blotting Analysis: A representative lot detected PF4 under both reduced and non-reduced condition by recognizing a linear epitope (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
Biological Information
ImmunogenPurified human platelet PF4 (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
Clone197.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 197.2 targets a linear epitope sensitive to heparin binding, but insensitive to reducing agent or denaturation by SDS (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PF4
  • CXCL4
  • SCYB4
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~8 kDa (recombinant mature hPF4) observed. 10.84 kDa (pro-form), 7.769 kDa (mature; a.a. 32-101), 6.033 kDa (cleaved short form; a.a. 48-101) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in human platelets.

Flow Cytometry Analysis: 1 µg of this antibody detected PF4 in one million 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized human platelets.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABS1255 04054839056321