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ABN1681 Anti-Phospho-PANX1 (Tyr198)

Anti-Phospho-PANX1 (Tyr198), Cat. No. ABN1681, is a highly specific rabbit polyclonal antibody that targets Pannexin 1 and has been tested in Immunofluorescence and Western blotting.

Anti-Phospho-PANX1 (Tyr198) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Panx1 UniProt Number: Q9JIP4
ABN1681
100 µL  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IFRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABN1681
DescriptionAnti-Phospho-PANX1 (Tyr198)
Alternate Names
  • Pannexin-1
Background InformationPannexin-1 (UniProt: Q9JIP4) is encoded by the Panx1 gene (Gene ID: 55991) in murine species. Pannexin-1 is a multi-pass membrane protein of the pannexin family that serves as a high-conductance, voltage-gated channel. It is widely expressed, including in cartilage, skin, spleen, heart, and brain. In the nervous system Panx1 transcripts have been detected in the cerebellum, cortex, lens, retina, pyramidal cells, interneurons of the neocortex and hippocampus. Three pannexin family genes (PANX1-3) have been identified in murine and human genomes. Pannexins share structural and functional similarities and play important roles in several physiological processes, including ATP release, Ca2+ homeostasis, and synaptic neurotransmitters release. Pannexins can also form intermixed channels and the intermixing is more evident between Pannexin 1 and 2. The level of intermixing is governed by the degree of N-glycosylation. Post-translational N-glycosylation plays a role in cell surface targeting. Pannexin-1 can also undergo S-nitrosylation, which can inhibit channel currents and ATP release. It has been shown that Pannexin-1 channel can release ATP in response to activation by TNF-alpha that requires activation of type-1 TNF receptors and downstream signaling through Src family kinases (SFK). Tryosine 198 in the intracellular loop region of Pannexin-1 is highly conserved and is considered as a SFK phosphorylation site. Pannexin-1 has been linked to propagation of calcium waves, regulation of vascular tone, taste-bud function, and in tumor suppression in gliomas. Pannexin-1 channel opening can contribute to cell death and seizures under ischemic or epileptic conditions. (Ref.: Penuela, S et al. (2013). Biochim. Biophys. Acta 1828(11); 15-22).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 8.0), 0.1% BSA without azide.
Quality LevelMQ100
Applications
ApplicationAnti-Phospho-PANX1 (Tyr198), Cat. No. ABN1681, is a highly specific rabbit polyclonal antibody that targets Pannexin 1 and has been tested in Immunofluorescence and Western blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
Application NotesWestern Blotting Analysis: A representative lot detected Phospho-PANX1 (Tyr198) in human coronary smooth muscle cells transfected with an epitope tagged Panx1, & recombinant human Pannexin1 and (active) human Src tyrosine kinase. (Courtesy of Isakson Lab, University of Virginia, Charlottesville, VA).

Immunofluorescence Analysis: A representative lot detected Phospho-PANX1 (Tyr198) in isolated mesenteric venule cross-sections from mice (Lohman, A.W., et. al. (2015). Nat. Commun. 6:7965).

Western Blotting Analysis: A representative lot detected Phospho-PANX1 (Tyr198) in HUVECs stimulated with TNF-a (Lohman, A.W., et. al. (2015). Nat Commun. 6:7965).
Biological Information
ImmunogenA linear peptide corresponding to 12 amino acids surrounding phospho-Tyr198 of murine Pannexin-1.
Epitopecytoplasmic domain
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody specifically detects pannexin-1 phosphorylated on tyrosine 198 in HEK293 cells transfected with murine Pannexin 1.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse and Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Panx1
Modifications
  • Phosphorylation
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~58 kDa observed; 48.17 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in transfected HEK293 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Phospho-PANX1 (Tyr198) in 10 µg of lysate from HEK293 cells transfected with murine pannexin-1.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ABN1681 04054839176579