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ABN1367 Anti-Pan DIDO Antibody

This Anti-Pan DIDO Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of DIDO.

Anti-Pan DIDO Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: DIDO1, C20orf158, DATF1, KIAA0333 UniProt Number: Q9BTC0
ABN1367
100 μg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN1367
DescriptionAnti-Pan DIDO Antibody
Alternate Names
  • Death-inducer obliterator 1
  • DATF-1
  • Death-associated transcription factor 1
  • DIDO1
  • DIDO2
  • DIDO3
  • DIO-1
  • hDido1
  • hDido2
  • hDido3
Background InformationDeath-inducer obliterator 1 (UniProt Q9BTC0; also known as DATF-1, Death-associated transcription factor 1, DIO-1, hDido1) is encoded by the DIDO1 (also known as C20orf158, DATF1, KIAA0333) gene (Gene ID 11083) in human. The DIDO1 gene encodes four alternatively spliced variants (DIDO1/isoform 2, DIDO2/isoform 1, DIDO3/isoform 4, isoform a/isoform 3) implicated to be involved in apoptosis and development. The longest and most widely expressed DIDO3 is assigned as the canonical isoform. DIDO3 is a nuclear protein that forms part of the spindle assembly checkpoint (SAC) and is necessary for correct chromosome segregation in somatic and germ cells. Dido3-knockout compromises differentiation of murine embryonic stem cells (mESCs) in vitro and of epiblast cells in vivo, resulting in early embryonic death at around day 8.5 of gestation. On the other hand, exogenous expression of Dido1 isoform in mESCs is reported to inhibit LIF withdrawal-induced differentiation, while Dido1 knockdown facilitate mESCs differentiation.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Pan DIDO Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of DIDO.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected DIDO immunoreactivity in human kindey, human prostate cancer and human cerebellum tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding an N-terminal sequence of human DIDO1.
EpitopeN-terminal region.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects an N-terminal epitope present in all four spliced isoforms of human DIDO1 reported by UniProt (Q9BTC0).
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse, Xenopus, Non-Human Primate based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • DIDO1
  • C20orf158
  • DATF1
  • KIAA0333
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~260/130/79/70/66 observed. 59.46 kDa (isoform 2; DIDO1), 129.2 kDa (isoform 1; DIDO2), 243.9 kDa (isoform 4; DIDO3), 61.35 kDa (isoform 3; isoform a) calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected DIDO isoforms in 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ABN1367 04055977351484