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07-1218 Anti-PP1γ1 Antibody

07-1218
100 µg  
Purchase on Sigma-Aldrich

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Áttekintés

Replacement Information
Replacement Information07-1218 is a recommended replacement for AB4084

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Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, H, M, RWB, IPRbPurifiedPolyclonal Antibody
Description
Catalogue Number07-1218
ReplacesAB4084
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-PP1γ1 Antibody
Alternate Names
  • Protein phosphatase 1C catalytic subunit
  • Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
  • Protein phosphatase 1, catalytic subunit, gamma isoform
  • Serine/threonine phosphatase 1 gamma
Background InformationType 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • HeLa Cell Lysate
PresentationPurified rabbit polyclonal IgG provided as 0.2µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PP1γ1 Antibody detects level of PP1γ1 & has been published & validated for use in WB & IP.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation: A previous lot of this antibody was used on Immunoprecipitation.
Biological Information
ImmunogenThe immunogen used to the purified peptide conjugated to KLH corresponding to the to the C –terminus of PP1 gamma 1 catalytic subunit (amino acids 311-323).
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes Protein Phosphatase 1γ1. No cross reactivity with other recombinant PP1 isoforms.
IsotypeIgG
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PPP1CC
  • PP-1G
  • PPP1G
  • EC 3.1.3.16
Purification MethodPurified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P36873 # Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II.
COFACTOR: Binds 1 iron ion per subunit. & Binds 1 manganese ion per subunit.
SIZE: 323 amino acids; 36984 Da
SUBUNIT: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. PPP1R12A and PPP1R12B mediate binding to myosin. PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen. Interacts with cyanobacterial toxin microcystin; disulfide-linked. Interacts with PPP1R7. Isoform gamma-2 interacts with SPZ1 (By similarity). Interacts with CDCA2.
SUBCELLULAR LOCATION: Cytoplasm. Note=Colocalizes with SPZ1 in the nucleus (By similarity).
SIMILARITY: SwissProt: P36873 ## Belongs to the PPP phosphatase family. PP-1 subfamily.
MISCELLANEOUS: Microcystin toxin is bound to Cys-273 through a thioether bond.
Molecular Weight~37 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis: 1:500 dilution of this lot detected PP-1 GAMMA on 10 μg of HeLa lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
07-1218 04053252738289

Documentation

Anti-PP1γ1 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-PP1γ1 Antibody Certificates of Analysis

TitleLot Number
Anti-PP11 - 2051935 2051935
Anti-PP11 - 2273994 2273994
Anti-PP11 - JBC1785478 JBC1785478
Anti-PP11 - NG1602926 NG1602926
Anti-PP11 - NG1660337 NG1660337
Anti-PP11 - NG1876737 NG1876737
Anti-PP11 - NG1948814 NG1948814
Anti-PP1γ1 - 3763381 3763381
Anti-PP1γ1 - 3915603 3915603
Anti-PP1γ1 -2567065 2567065

References

Reference overviewPub Med ID
High NaCl-induced inhibition of PTG contributes to activation of NFAT5 through attenuation of the negative effect of SHP-1.
Zhou, X; Wang, H; Burg, MB; Ferraris, JD
American journal of physiology. Renal physiology  305  F362-9  2013

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