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DR1108 Anti-POLI Mouse mAb (8G9)

Anti-POLI Mouse mAb (8G9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Polymerase DNA-Directed ι

DR1108
  
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Áttekintés

Replacement Information

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Description
Overview

This product has been discontinued.





Recognizes POLI protein in cell lysates.
Catalogue NumberDR1108
Brand Family Calbiochem®
SynonymsAnti-Polymerase DNA-Directed ι
Application Data
Detection of human POLI by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-POLI Mouse mAb (8G9) (Cat. No. DR1108) (1-5 µg/ml). Detection: chemilimunescence.
References
ReferencesWang, Y., et al. 2007. Cancer Res. 67 3018.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlHeLa NE cells
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see coments) Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 616-716 human POLI, expressed as a GST fusion protein
Clone8G9
HostMouse
IsotypeIgG₃
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
DR1108 0

Documentation

References

Hivatkozások áttekintése
Wang, Y., et al. 2007. Cancer Res. 67 3018.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-November-2010 RFH
SynonymsAnti-Polymerase DNA-Directed ι
ApplicationELISA (see coments) Immunoblotting (1-5 µg/ml)
Application Data
Detection of human POLI by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-POLI Mouse mAb (8G9) (Cat. No. DR1108) (1-5 µg/ml). Detection: chemilimunescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~75 kDa POLI protein.
BackgroundError-prone, DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 616-716 human POLI, expressed as a GST fusion protein
Clone8G9
IsotypeIgG₃
Specieshuman
Positive controlHeLa NE cells
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesWang, Y., et al. 2007. Cancer Res. 67 3018.