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MABC1720-25UG Anti-PMEL Antibody, clone HMB-45

Anti-PMEL, clone HMB-45, Cat. No. MABC1720, is a mouse monoclonal antibody that detects Melanocyte protein PMEL (HMB-45) and has been tested for use in Immunohistochemistry (Paraffin).

MABC1720-25UG
25 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberMABC1720-25UG
DescriptionAnti-PMEL Antibody, clone HMB-45
Alternate Names
  • Melanocyte protein PMEL
  • ME20-M
  • ME20M
  • Melanocyte protein Pmel 17
  • Melanocytes lineage-specific antigen GP100
  • Melanoma-associated ME20 antigen
  • P1
  • P100
  • Premelanosome protein
  • Silver locus protein homolog
Background InformationMelanocyte protein PMEL (UniProt: P40967; also known as ME20-M, ME20M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog) is encoded by the PMEL (also known as D12S53E, PMEL17, SILV) gene (Gene ID: 6490) in human. PMEL is a disulfide-linked heterodimeric glycoprotein protein composed of a soluble M-alpha (aa 24-467; ME20S) and an integral membrane subunit M-beta (aa 470-661). It plays a key role in the biogenesis and maturation of melanosome from stage I to stage II. It is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to produce the mature form, which then cleaves into M-alpha and M-beta chains. It has been shown that despite cleavage, only a small fraction of M-alpha is secreted, whereas most M-alpha and M-beta remain associated with each other intracellularly. PMEL is preferentially expressed in melanomas and is neither found in normal tissues nor in carcinomas. In quiescent adult melanocytes it is found in very low levels. The release of soluble form is reported to protect tumor cells from antibody-mediated immunity. Five different isoforms of PMEL have been described that are produced by alternative splicing.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-PMEL, clone HMB-45, Cat. No. MABC1720, is a mouse monoclonal antibody that detects Melanocyte protein PMEL (HMB-45) and has been tested for use in Immunohistochemistry (Paraffin).
Key Applications
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected PMEL in human melanoma tissue sections.

Immunohistochemistry (Paraffin) Analysis: A representative lot detected PMEL in Immunohistochemistry applications (Gown, A.M., et. al. (1986). Am J Pathol. 123(2):195-203).
Biological Information
ImmunogenPigmented melanoma metastases extracted from human lymph nodes.
CloneHMB-45
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone HMB-45 is a mouse monoclonal antibody that detects human Melanocyte protein PMEL.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PMEL
  • D12S53E
  • PMEL17
Purification MethodProtein G purified
UniProt Number
Molecular Weight70.25 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human skin tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected PMEL in human skin tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABC1720-25UG 04054839007217

Documentation

Anti-PMEL Antibody, clone HMB-45 Certificates of Analysis

TitleLot Number
Anti-PMEL, clone HMB-45 - 3245722 3245722

References

Reference overviewPub Med ID
Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes.
Gown, AM; Vogel, AM; Hoak, D; Gough, F; McNutt, MA
Am J Pathol  124  195-204  1987

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