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MABN842 Anti-PERK Antibody, clone 4F6.1

This Anti-PERK Antibody, clone 4F6.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of PERK.

Anti-PERK Antibody, clone 4F6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: EIF2AK3, PEK, PERK UniProt Number: Q9NZJ5
MABN842
100 μg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN842
DescriptionAnti-PERK Antibody, clone 4F6.1
Alternate Names
  • PERK
  • Eukaryotic translation initiation factor 2-alpha kinase 3
  • PRKR-like endoplasmic reticulum kinase
  • Pancreatic eIF2-alpha kinase
  • HsPEK
Background InformationEukaryotic translation initiation factor 2-alpha kinase 3 (EC 2.7.11.1; UniProt Q9NZJ5; also known as Eukaryotic translation initiation factor 2 alpha kinase 3, HsPEK, Pancreatic EIF2-alpha kinase, PRKR-like endoplasmic reticulum kinase) is encoded by the EIF2AK3 (also known as PEK, PERK, WRS) gene (Gene ID 9451) in human. The accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) induces the activation of three main unfold protein response (UPR) pathways, the inositol requiring enzyme-1 (IRE1) pathway, the activating transcription factor 6 (ATF6) pathway, and the protein kinase-like ER kinase (PERK) pathway. The PERK pathway involves PERK auto-phosphorylation/activation, resulting in PERK phosphorylation of eIF2α, which in turn leads to increased ATF4 transcription and ATF4-mediated CAAT/enhancer binding protein (C/EBP) homologous protein (CHOP) upregulation.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-PERK Antibody, clone 4F6.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of PERK.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PERK in human liver tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the Internal (C-terminal half) of human PERK.
EpitopeInternal (C-terminal half)
Clone4F6.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • EIF2AK3
  • PEK
  • PERK
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~125 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected PERK in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABN842 04055977181678

Documentation

Anti-PERK Antibody, clone 4F6.1 MSDS

Title

Safety Data Sheet (SDS) 

Anti-PERK Antibody, clone 4F6.1 Certificates of Analysis

TitleLot Number
Anti-PERK, clone 4F6.1 - 3291399 3291399
Anti-PERK, clone 4F6.1 - 3907009 3907009
Anti-PERK, clone 4F6.1 - 4142772 4142772
Anti-PERK, clone 4F6.1 -Q2586373 Q2586373
Anti-PERK, clone 4F6.1 Monoclonal Antibody 3068047
Anti-PERK, clone 4F6.1 Monoclonal Antibody 2990469

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.